CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005861
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 M-phase inducer phosphatase 2 
Protein Synonyms/Alias
 Dual specificity phosphatase Cdc25B 
Gene Name
 CDC25B 
Gene Synonyms/Alias
 CDC25HU2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
320LISAPLVKTLEKEEEubiquitination[1]
419ELIGDYSKAFLLQTVubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. 
Sequence Annotation
 DOMAIN 431 538 Rhodanese.
 ACT_SITE 487 487
 MOD_RES 169 169 Phosphoserine; by MELK.
 MOD_RES 249 249 Phosphoserine.
 MOD_RES 323 323 Phosphoserine; by MELK and MAPK14.
 MOD_RES 353 353 Phosphoserine; by AURKA.
 MOD_RES 375 375 Phosphoserine; by BRSK1 and MAPK14.  
Keyword
 3D-structure; Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Hydrolase; Mitosis; Phosphoprotein; Polymorphism; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 580 AA 
Protein Sequence
MEVPQPEPAP GSALSPAGVC GGAQRPGHLP GLLLGSHGLL GSPVRAAASS PVTTLTQTMH 60
DLAGLGSRSR LTHLSLSRRA SESSLSSESS ESSDAGLCMD SPSPMDPHMA EQTFEQAIQA 120
ASRIIRNEQF AIRRFQSMPV RLLGHSPVLR NITNSQAPDG RRKSEAGSGA ASSSGEDKEN 180
VRFWKAGVGA LREEEGACWG GSLACEDPPL PSWLQDGFVF KMPWKPTHPS STHALAEWAS 240
RREAFAQRPS SAPDLMCLSP DRKMEVEELS PLALGRFSLT PAEGDTEEDD GFVDILESDL 300
KDDDAVPPGM ESLISAPLVK TLEKEEEKDL VMYSKCQRLF RSPSMPCSVI RPILKRLERP 360
QDRDTPVQNK RRRSVTPPEE QQEAEEPKAR VLRSKSLCHD EIENLLDSDH RELIGDYSKA 420
FLLQTVDGKH QDLKYISPET MVALLTGKFS NIVDKFVIVD CRYPYEYEGG HIKTAVNLPL 480
ERDAESFLLK SPIAPCSLDK RVILIFHCEF SSERGPRMCR FIRERDRAVN DYPSLYYPEM 540
YILKGGYKEF FPQHPNFCEP QDYRPMNHEA FKDELKTFRL KTRSWAGERS RRELCSRLQD 600
Q 601 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0004725; F:protein tyrosine phosphatase activity; TAS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007144; P:female meiosis I; IEA:Compara.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:UniProtKB.
 GO:0007067; P:mitosis; TAS:ProtInc.
 GO:0001556; P:oocyte maturation; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0032467; P:positive regulation of cytokinesis; IMP:UniProtKB.
 GO:0045931; P:positive regulation of mitotic cell cycle; IMP:UniProtKB.
 GO:0045860; P:positive regulation of protein kinase activity; IEA:Compara.
 GO:0006468; P:protein phosphorylation; IDA:UniProtKB. 
Interpro
 IPR000751; MPI_Phosphatase.
 IPR001763; Rhodanese-like_dom. 
Pfam
 PF06617; M-inducer_phosp
 PF00581; Rhodanese 
SMART
 SM00450; RHOD 
PROSITE
 PS50206; RHODANESE_3 
PRINTS
 PR00716; MPIPHPHTASE.