CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004959
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Multidrug resistance protein 3 
Protein Synonyms/Alias
 ATP-binding cassette sub-family B member 4; Multidrug resistance protein 2; P-glycoprotein 2; P-glycoprotein 3 
Gene Name
 Abcb4 
Gene Synonyms/Alias
 Mdr2; Pgy-2; Pgy2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
241ILSTFSDKELAAYAKubiquitination[1]
278KELERYQKHLENAKKubiquitination[1]
388GHKPDNIKGNLEFSDubiquitination[1]
410RANIKILKGLNLKVKubiquitination[1]
417KGLNLKVKSGQTVALubiquitination[1]
449LYDPTEGKISIDGQDubiquitination[1]
501VTMDEIEKAVKEANAubiquitination[1]
504DEIEKAVKEANAYDFubiquitination[1]
514NAYDFIMKLPQKFDTubiquitination[1]
518FIMKLPQKFDTLVGDubiquitination[1]
574EVQAALDKAREGRTTubiquitination[1]
616GSHSELMKKEGIYFRubiquitination[1]
658DVAPNGWKARIFRNSubiquitination[1]
696VPPVSFLKVLKLNKTubiquitination[1]
919FESMYVEKLHGPYRNubiquitination[1]
1057QGLSLEVKKGQTLALubiquitination[1]
1098LLDGQEAKKLNVQWLubiquitination[1]
1099LDGQEAKKLNVQWLRubiquitination[1]
1146DEIVRAAKEANIHPFubiquitination[1]
1160FIETLPQKYNTRVGDubiquitination[1]
1216VVQEALDKAREGRTCubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. 
Sequence Annotation
 DOMAIN 54 356 ABC transmembrane type-1 1.
 DOMAIN 391 627 ABC transporter 1.
 DOMAIN 708 996 ABC transmembrane type-1 2.
 DOMAIN 1031 1269 ABC transporter 2.
 NP_BIND 426 433 ATP 1 (By similarity).
 NP_BIND 1066 1073 ATP 2 (By similarity).
 REGION 622 646 Interaction with HAX1 (By similarity).
 CARBOHYD 88 88 N-linked (GlcNAc...) (Potential).
 CARBOHYD 94 94 N-linked (GlcNAc...) (Potential).  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Glycoprotein; Hydrolase; Membrane; Nucleotide-binding; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1276 AA 
Protein Sequence
MDLEAARNGT ARRLDGDFEL GSISNQGREK KKKVNLIGLL TLFRYSDWQD KLFMFLGTLM 60
AIAHGSGLPL MMIVFGEMTD KFVDNTGNFS LPVNFSLSML NPGRILEEEM TRYAYYYSGL 120
GGGVLVAAYI QVSFWTLAAG RQIKKIRQKF FHAILRQEMG WFDIKGTTEL NTRLTDDVSK 180
ISEGIGDKVG MFFQAIATFF AGFIVGFIRG WKLTLVIMAI SPILGLSTAV WAKILSTFSD 240
KELAAYAKAG AVAEEALGAI RTVIAFGGQN KELERYQKHL ENAKKIGIKK AISANISMGI 300
AFLLIYASYA LAFWYGSTLV ISKEYTIGNA MTVFFSILIG AFSVGQAAPC IDAFANARGA 360
AYVIFDIIDN NPKIDSFSER GHKPDNIKGN LEFSDVHFSY PSRANIKILK GLNLKVKSGQ 420
TVALVGNSGC GKSTTVQLLQ RLYDPTEGKI SIDGQDIRNF NVRCLREIIG VVSQEPVLFS 480
TTIAENIRYG RGNVTMDEIE KAVKEANAYD FIMKLPQKFD TLVGDRGAQL SGGQKQRIAI 540
ARALVRNPKI LLLDEATSAL DTESEAEVQA ALDKAREGRT TIVIAHRLST IRNADVIAGF 600
EDGVIVEQGS HSELMKKEGI YFRLVNMQTA GSQILSEEFE VELSDEKAAG DVAPNGWKAR 660
IFRNSTKKSL KSPHQNRLDE ETNELDANVP PVSFLKVLKL NKTEWPYFVV GTVCAIANGA 720
LQPAFSIILS EMIAIFGPGD DAVKQQKCNM FSLVFLGLGV LSFFTFFLQG FTFGKAGEIL 780
TTRLRSMAFK AMLRQDMSWF DDHKNSTGAL STRLATDAAQ VQGATGTRLA LIAQNTANLG 840
TGIIISFIYG WQLTLLLLSV VPFIAVAGIV EMKMLAGNAK RDKKEMEAAG KIATEAIENI 900
RTVVSLTQER KFESMYVEKL HGPYRNSVRK AHIYGITFSI SQAFMYFSYA GCFRFGSYLI 960
VNGHMRFKDV ILVFSAIVLG AVALGHASSF APDYAKAKLS AAYLFSLFER QPLIDSYSGE 1020
GLWPDKFEGS VTFNEVVFNY PTRANVPVLQ GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE 1080
RFYDPMAGSV LLDGQEAKKL NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVPHDE 1140
IVRAAKEANI HPFIETLPQK YNTRVGDKGT QLSGGQKQRI AIARALIRQP RVLLLDEATS 1200
ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIENGKVKEH GTHQQLLAQK 1260
GIYFSMVNIQ AGTQNL 1276 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IBA:RefGenome.
 GO:0000139; C:Golgi membrane; IBA:RefGenome.
 GO:0016021; C:integral to membrane; IBA:RefGenome.
 GO:0046581; C:intercellular canaliculus; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042626; F:ATPase activity, coupled to transmembrane movement of substances; IBA:RefGenome.
 GO:0008559; F:xenobiotic-transporting ATPase activity; IEA:EC. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS