CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019193
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 THAP domain-containing protein 11 
Protein Synonyms/Alias
  
Gene Name
 THAP11 
Gene Synonyms/Alias
 HRIHFB2206 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19YNNSHRDKALHFYTFubiquitination[1, 2, 3]
28LHFYTFPKDAELRRLubiquitination[2, 3]
38ELRRLWLKNVSRAGVubiquitination[1, 2, 4, 5, 6]
276ALMEVKMKEMKGSIRubiquitination[2]
291HLRLTEAKLREELREubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers (By similarity). 
Sequence Annotation
 ZN_FING 1 80 THAP-type.
 MOTIF 243 246 HCFC1-binding motif (HBM) (By  
Keyword
 3D-structure; Coiled coil; Complete proteome; Cytoplasm; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation; Triplet repeat expansion; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 314 AA 
Protein Sequence
MPGFTCCVPG CYNNSHRDKA LHFYTFPKDA ELRRLWLKNV SRAGVSGCFS TFQPTTGHRL 60
CSVHFQGGRK TYTVRVPTIF PLRGVNERKV ARRPAGAAAA RRRQQQQQQQ QQQQQQQQQQ 120
QQQQQQQQQQ QQSSPSASTA QTAQLQPNLV SASAAVLLTL QATVDSSQAP GSVQPAPITP 180
TGEDVKPIDL TVQVEFAAAE GAAAAAAASE LQAATAGLEA AECPMGPQLV VVGEEGFPDT 240
GSDHSYSLSS GTTEEELLRK LNEQRDILAL MEVKMKEMKG SIRHLRLTEA KLREELREKD 300
RLLAMAVIRK KHGM 314 
Gene Ontology
 GO:0005825; C:half bridge of spindle pole body; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR006612; Znf_C2CH. 
Pfam
 PF05485; THAP 
SMART
 SM00692; DM3
 SM00980; THAP 
PROSITE
 PS50950; ZF_THAP 
PRINTS