CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018328
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gamma-secretase subunit APH-1B 
Protein Synonyms/Alias
 APH-1b; Aph-1beta; Presenilin-stabilization factor-like 
Gene Name
 APH1B 
Gene Synonyms/Alias
 PSFL; UNQ688/PRO1328 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
92MFRFAYYKLLKKASEubiquitination[1, 2]
102KKASEGLKSINPGETubiquitination[3]
247LCLLCQDKNFLLYNQubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A. 
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Membrane; Notch signaling pathway; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 257 AA 
Protein Sequence
MTAAVFFGCA FIAFGPALAL YVFTIATEPL RIIFLIAGAF FWLVSLLISS LVWFMARVII 60
DNKDGPTQKY LLIFGAFVSV YIQEMFRFAY YKLLKKASEG LKSINPGETA PSMRLLAYVS 120
GLGFGIMSGV FSFVNTLSDS LGPGTVGIHG DSPQFFLYSA FMTLVIILLH VFWGIVFFDG 180
CEKKKWGILL IVLLTHLLVS AQTFISSYYG INLASAFIIL VLMGTWAFLA AGGSCRSLKL 240
CLLCQDKNFL LYNQRSR 257 
Gene Ontology
 GO:0016021; C:integral to membrane; IDA:MGI.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0030133; C:transport vesicle; IDA:LIFEdb.
 GO:0008233; F:peptidase activity; IDA:MGI.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0031293; P:membrane protein intracellular domain proteolysis; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0007220; P:Notch receptor processing; TAS:Reactome.
 GO:0007219; P:Notch signaling pathway; TAS:Reactome.
 GO:0043085; P:positive regulation of catalytic activity; IEA:InterPro.
 GO:0016485; P:protein processing; IDA:MGI. 
Interpro
 IPR009294; Aph-1. 
Pfam
 PF06105; Aph-1 
SMART
  
PROSITE
  
PRINTS