CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013917
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasome activator complex subunit 4 
Protein Synonyms/Alias
 Proteasome activator PA200; Protein TEMO 
Gene Name
 Psme4 
Gene Synonyms/Alias
 Kiaa0077 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
56DLQLAQIKSNLGRAVubiquitination[1]
355RWLNKLMKLLQRLPNacetylation[2]
916HEFDSRWKSFNLVKKubiquitination[1]
1082LFDDLAEKIHRQYETubiquitination[1]
1124QTLLSPEKIKEGQKRubiquitination[1]
1294AGVEEQPKLGRSREDubiquitination[1]
1346PRRFCLFKGIFRNFDacetylation[2]
1361DAFLPVLKPHLERLVubiquitination[1]
1488ELLHRLLKYLEPKLTacetylation[2]
1488ELLHRLLKYLEPKLTubiquitination[1]
1499PKLTQVYKNVRERIGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin- independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks. 
Sequence Annotation
 REPEAT 475 519 HEAT 1.
 REPEAT 998 1037 HEAT 2.
 REPEAT 1179 1217 HEAT 3.
 REPEAT 1354 1392 HEAT 4.
 REPEAT 1636 1674 HEAT 5.
 REPEAT 1680 1718 HEAT 6.
 REGION 1650 1738 Bromodomain-like (BRDL) (By similarity).
 MOD_RES 1121 1121 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Differentiation; DNA damage; DNA repair; Nucleus; Phosphoprotein; Proteasome; Reference proteome; Repeat; Spermatogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1843 AA 
Protein Sequence
MEAPERAGGG EPPEPGGRPV LGPRAFVPQK EIVYNKLLPY AERLDAESDL QLAQIKSNLG 60
RAVQLQELWP GGLFWTRKLS TYIRLYGRKF SKEDHVLFIK LLYELVSIPK LEISMMQGFA 120
RLLINLLKKK ELLSRDDLEL PWRPLYDLVE RILYSKTEHL RLNSFPNSIE NVLKTLVKSC 180
RPYFPADSTA EMLEEWRPLM CPFDVTMQKA ISYFEIFLPT SLPPELHHKG FKLWFDELIG 240
LWVSVQNLPQ WEGQLVNLFA RLATDNIGYI DWDPYVPKIF TRILRSLNLP VGSSQVLVPR 300
FLTNAYDIGH AVIWITAMMG GPSKLVQKHL AGLFNSITSF YHPSNNGRWL NKLMKLLQRL 360
PNSVVRRLHR ERFKKPSWLT PVPESHKLTD EDVTDFVQCI IQPVLLAMFS KTGSLEAAQA 420
LQNLALMRPE LVIPPVLERT YPALETLTEP HQLTATLNCV IGVARSLVSG SKWFPEGPTH 480
MLPLLMRALP GVDPNDFSKC MITFQFIGTF STLVPLVDCS SVLQERNDLT EIEKELCSAT 540
AEFEDFVLQF MDRCFGLIES STLEQTREET ETEKMTHLES LVELGLSSTF STILTQCSKD 600
IFMVALQKVF NFSVSHIFET RVAGRMVADM CRAAVKCCPE ESLKLFVPHC YGVITQLTMN 660
DDVLNEEELD KELLWNLQLL SEITRVDGKK LLLYREQLVK ILQRTLHLTC KQGYTLSCNL 720
LHHLLRSTTL IYPTEYCSVP GGFNKPPSEY FPVKDWGKPG DLWNLGIQWH VPSSEEVSFA 780
FYLLDSFLQP ELIKLQCCGD GELEMSRDDI LQSLTIVHSC LIGSGNLLPP LKGEAVTNLV 840
PSMVSLEETK LYTGLEHDLS RENYREVIAS VIRKLLSHIL DNSEDDTKSL FLIIKIIGDL 900
LHFQGSHKHE FDSRWKSFNL VKKSMENRLH GKKQHIRALL IDRVMLQHEL RTLTVEGCEY 960
KKIHQDMIRD LLRLSTSSYS QVRNKAQQTF FAALGAYNFC CRDIIPLVLE FLRPDRKDVT 1020
QQQFKGALYC LLGNHSGVCL ANLHDWDCIV QTWPALVSSG LSQAMSLEKP SIVRLFDDLA 1080
EKIHRQYETI GLDFTIPKSC AAIAELLQQS KNPSISQTLL SPEKIKEGQK RQQDKNADAL 1140
RNYECLVNTL LDGVEQRNLP WKFEHIGIGL LSLLLRDDRV LPLRAIRFFV ENLNHDAIVV 1200
RKMAISAVAG ILKQLKRTHK KLTINPYEIS GCPKPTKILA GDRPDNHWLH YDSKNIPRTK 1260
KEWESSCFVE KTHWGYYNWP KNMVVYAGVE EQPKLGRSRE DMIEAEQIIY DRFSDPKFVE 1320
QLITFLSLED RKGRDKFSPR RFCLFKGIFR NFDDAFLPVL KPHLERLVAD SHESTQRCVA 1380
EIIAGLIRGS KHWTFEKVEK LWELLCPLLR TALSNMTVET YNDWGTCIAT SCESRDPRKL 1440
HWLFELLLES PLSGEGGSFV DACRLYVLQG GLAQQEWRVP ELLHRLLKYL EPKLTQVYKN 1500
VRERIGSVLT YIFMIDVSLP NTAPTTSPCI PEFTARVLEK LKPLTDVDEE IQNHVMEENG 1560
IGEEDERTQG IKLLKTILKW LMASAGRSFS TAVKEQLQLL PLFFKIAPVE NDNSYDELKR 1620
DAKLCLSLMS QGLLYPQQVP LILQVLSQTA RSSSWHARYT VLTYLQTMVF YNLFIFLNNE 1680
DAVKDIRWLI ICLLEDEQLE VREMAATTLS GLLQCNFLTM DSAMQIHFEQ LCKTKLPKKR 1740
KRDPGSVGDT IPSAELVKRH AGVLGLGACV LSSPYDVPTW MPQLLMNLSA HLNDPQPIEM 1800
TVKKTLSNFR RTHHDNWQEH KQQFTDDQLL VLTDLLVSPC YYA 1843 
Gene Ontology
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021843; DUF3437. 
Pfam
 PF11919; DUF3437 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS