CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009201
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA pseudouridine synthase B 
Protein Synonyms/Alias
 Protein p35; tRNA pseudouridine(55) synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase 
Gene Name
 truB 
Gene Synonyms/Alias
 yhbA; b3166; JW3135 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
31SSNDALQKVKRIYNAacetylation[1]
74QYLLDSDKRYRVIARacetylation[1]
190IIDDLGEKLGCGAHVacetylation[1]
207LRRLAVSKYPVERMVacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs. 
Sequence Annotation
 REGION 124 152 RNA binding (By similarity).
 ACT_SITE 48 48 Nucleophile.
 BINDING 43 43 Substrate.
 BINDING 76 76 Substrate.
 BINDING 179 179 Substrate (Probable).
 BINDING 202 202 Substrate.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Isomerase; Reference proteome; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 314 AA 
Protein Sequence
MSRPRRRGRD INGVLLLDKP QGMSSNDALQ KVKRIYNANR AGHTGALDPL ATGMLPICLG 60
EATKFSQYLL DSDKRYRVIA RLGQRTDTSD ADGQIVEERP VTFSAEQLAA ALDTFRGDIE 120
QIPSMYSALK YQGKKLYEYA RQGIEVPREA RPITVYELLF IRHEGNELEL EIHCSKGTYI 180
RTIIDDLGEK LGCGAHVIYL RRLAVSKYPV ERMVTLEHLR ELVEQAEQQD IPAAELLDPL 240
LMPMDSPASD YPVVNLPLTS SVYFKNGNPV RTSGAPLEGL VRVTEGENGK FIGMGEIDDE 300
GRVAPRRLVV EYPA 314 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IMP:EcoCyc.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0001522; P:pseudouridine synthesis; IDA:EcoCyc.
 GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP. 
Interpro
 IPR002501; PsdUridine_synth.
 IPR020103; PsdUridine_synth_cat_dom.
 IPR015947; PUA-like_domain.
 IPR014780; tRNA_psdUridine_synth_TruB.
 IPR015240; tRNA_sdUridine_synth_fam1_C. 
Pfam
 PF09157; TruB-C_2
 PF01509; TruB_N 
SMART
  
PROSITE
  
PRINTS