UniProt Accession

 GNAI1_HUMAN; P63096; A8KA88; B4E2V1; C9J3A4; P04898; P11015; P31871; Q5U074; Q8TAN5; Q9UGA4 

Genbank Protein ID

 AF493905; AF055013; AL049933; BT019775; AK292953; AK304442; AC004159; AC080066; CH471091; BC026326; M20596; M20594; M20595; M17219 

Genbank Nucleotide ID

 AAM12619.1; AAC09361.1; CAB43212.2; AAV38580.1; BAF85642.1; BAG65263.1; EAW77011.1; AAH26326.1; AAA35893.1; AAA35893.1; AAA35893.1; AAA52581.1 

Protein Name

 Guanine nucleotide-binding protein G(i) subunit alpha-1 

Protein Synonyms/Alias

 Adenylate cyclase-inhibiting G alpha protein 

Gene Name

 GNAI1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
29	NLREDGEKAAREVKL	ubiquitination	[1]
35	EKAAREVKLLLLGAG	ubiquitination	[1, 2]
46	LGAGESGKSTIVKQM	ubiquitination	[2]
51	SGKSTIVKQMKIIHE	ubiquitination	[1]
92	IRAMGRLKIDFGDSA	ubiquitination	[1, 2, 3]
132	GVIKRLWKDSGVQAC	ubiquitination	[2]
192	VETHFTFKDLHFKMF	ubiquitination	[1, 3]
197	TFKDLHFKMFDVGGQ	ubiquitination	[1, 2, 3]
248	NRMHESMKLFDSICN	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. 

Sequence Annotation

 NP_BIND 40 47 GTP.
 NP_BIND 175 181 GTP (By similarity).
 NP_BIND 200 204 GTP.
 NP_BIND 269 272 GTP.
 METAL 47 47 Magnesium (By similarity).
 METAL 181 181 Magnesium (By similarity).
 BINDING 326 326 GTP; via amide nitrogen (By similarity).
 MOD_RES 178 178 ADP-ribosylarginine; by cholera toxin (By
 MOD_RES 351 351 ADP-ribosylcysteine; by pertussis toxin
 LIPID 2 2 N-myristoyl glycine.
 LIPID 3 3 S-palmitoyl cysteine (By similarity).  

Keyword

 3D-structure; ADP-ribosylation; Alternative splicing; Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Nucleus; Palmitate; Reference proteome; Transducer. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 354 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0031821; F:G-protein coupled serotonin receptor binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; ISS:BHF-UCL.
 GO:0003924; F:GTPase activity; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0007193; P:adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway; TAS:Reactome.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IMP:UniProtKB.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0043434; P:response to peptide hormone stimulus; ISS:BHF-UCL.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 

Interpro

 IPR001408; Gprotein_alpha_I.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00503; G-alpha 

SMART

 SM00275; G_alpha 

PROSITE

  

PRINTS

 PR00318; GPROTEINA.
 PR00441; GPROTEINAI.