CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001062
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase ULK1 
Protein Synonyms/Alias
 Serine/threonine-protein kinase Unc51.1; Unc-51-like kinase 1 
Gene Name
 Ulk1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
162NPSNIRVKIADFGFAacetylation[1]
606RPLRGSPKLPDFLQRacetylation[1]
Reference
 [1] GSK3-TIP60-ULK1 signaling pathway links growth factor deprivation to autophagy.
 Lin SY, Li TY, Liu Q, Zhang C, Li X, Chen Y, Zhang SM, Lian G, Liu Q, Ruan K, Wang Z, Zhang CS, Chien KY, Wu J, Li Q, Han J, Lin SC.
 Science. 2012 Apr 27;336(6080):477-81. [PMID: 22539723] 
Functional Description
 Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. 
Sequence Annotation
 DOMAIN 16 278 Protein kinase.
 NP_BIND 22 30 ATP (By similarity).
 ACT_SITE 138 138 Proton acceptor (By similarity).
 BINDING 46 46 ATP (Probable).
 MOD_RES 317 317 Phosphoserine; by AMPK.
 MOD_RES 450 450 Phosphoserine (By similarity).
 MOD_RES 456 456 Phosphothreonine (By similarity).
 MOD_RES 467 467 Phosphoserine.
 MOD_RES 477 477 Phosphoserine (By similarity).
 MOD_RES 479 479 Phosphoserine (By similarity).
 MOD_RES 555 555 Phosphoserine; by AMPK.
 MOD_RES 574 574 Phosphothreonine.
 MOD_RES 624 624 Phosphothreonine.
 MOD_RES 637 637 Phosphoserine; by AMPK.
 MOD_RES 757 757 Phosphoserine; by MTOR.
 MOD_RES 777 777 Phosphoserine; by AMPK.  
Keyword
 ATP-binding; Autophagy; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1051 AA 
Protein Sequence
MEPGRGGVET VGKFEFSRKD LIGHGAFAVV FKGRHREKHD LEVAVKCINK KNLAKSQTLL 60
GKEIKILKEL KHENIVALYD FQEMANSVYL VMEYCNGGDL ADYLHTMRTL SEDTVRLFLQ 120
QIAGAMRLLH SKGIIHRDLK PQNILLSNPG GRRANPSNIR VKIADFGFAR YLQSNMMAAT 180
LCGSPMYMAP EVIMSQHYDG KADLWSIGTI VYQCLTGKAP FQASSPQDLR LFYEKNKTLV 240
PAIPRETSAP LRQLLLALLQ RNHKDRMDFD EFFHHPFLDA STPIKKSPPV PVPSYPSSGS 300
GSSSSSSSAS HLASPPSLGE MPQLQKTLTS PADAAGFLQG SRDSGGSSKD SCDTDDFVMV 360
PAQFPGDLVA EAASAKPPPD SLLCSGSSLV ASAGLESHGR TPSPSPTCSS SPSPSGRPGP 420
FSSNRYGASV PIPVPTQVHN YQRIEQNLQS PTQQQTARSS AIRRSGSTTP LGFGRASPSP 480
PSHTDGAMLA RKLSLGGGRP YTPSPQVGTI PERPSWSRVP SPQGADVRVG RSPRPGSSVP 540
EHSPRTTGLG CRLHSAPNLS DFHVVRPKLP KPPTDPLGAT FSPPQTSAPQ PCPGLQSCRP 600
LRGSPKLPDF LQRSPLPPIL GSPTKAGPSF DFPKTPSSQN LLTLLARQGV VMTPPRNRTL 660
PDLSEASPFH GQQLGSGLRP AEDTRGPFGR SFSTSRITDL LLKAAFGTQA SDSGSTDSLQ 720
EKPMEIAPSA GFGGTLHPGA RGGGASSPAP VVFTVGSPPS GATPPQSTRT RMFSVGSSSS 780
LGSTGSSSAR HLVPGACGEA PELSAPGHCC SLADPLAANL EGAVTFEAPD LPEETLMEQE 840
HTETLHSLRF TLAFAQQVLE IAALKGSASE AAGGPEYQLQ ESVVADQISQ LSREWGFAEQ 900
LVLYLKVAEL LSSGLQTAID QIRAGKLCLS STVKQVVRRL NELYKASVVS CQGLSLRLQR 960
FFLDKQRLLD GIHGVTAERL ILSHAVQMVQ SAALDEMFQH REGCVPRYHK ALLLLEGLQH 1020
TLTDQADIEN IAKCKLCIER RLSALLSGVY A 1051 
Gene Ontology
 GO:0005776; C:autophagic vacuole; IEA:Compara.
 GO:0030659; C:cytoplasmic vesicle membrane; IDA:MGI.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0043005; C:neuron projection; IDA:MGI.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0034045; C:pre-autophagosomal structure membrane; IDA:UniProtKB.
 GO:0070969; C:ULK1-ATG13-FIP200 complex; IPI:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0000045; P:autophagic vacuole assembly; IMP:UniProtKB.
 GO:0048675; P:axon extension; IMP:MGI.
 GO:0021707; P:cerebellar granule cell differentiation; IMP:MGI.
 GO:0048671; P:negative regulation of collateral sprouting; IMP:MGI.
 GO:0031102; P:neuron projection regeneration; IEA:Compara.
 GO:0010508; P:positive regulation of autophagy; IMP:UniProtKB.
 GO:0016239; P:positive regulation of macroautophagy; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IDA:MGI.
 GO:0008104; P:protein localization; ISS:UniProtKB.
 GO:0021933; P:radial glia guided migration of cerebellar granule cell; IMP:MGI.
 GO:0007265; P:Ras protein signal transduction; IDA:MGI.
 GO:0031623; P:receptor internalization; IMP:MGI.
 GO:0051386; P:regulation of neurotrophin TRK receptor signaling pathway; IMP:MGI. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR016237; Ser/Thr_kin_STPK_Ulk-1/2.
 IPR008271; Ser/Thr_kinase_AS.
 IPR022708; Ser/Thr_kinase_C. 
Pfam
 PF12063; DUF3543
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS