CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000935
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fatty acid desaturase 1 
Protein Synonyms/Alias
 Delta(5) fatty acid desaturase; D5D; Delta(5) desaturase; Delta-5 desaturase 
Gene Name
 FADS1 
Gene Synonyms/Alias
 FADSD5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
98RWLVIDRKVYNISEFubiquitination[1, 2, 3, 4]
135FVAFHINKGLVKKYMubiquitination[3, 4, 5, 6]
139HINKGLVKKYMNSLLubiquitination[3]
140INKGLVKKYMNSLLIubiquitination[1, 2, 3, 4, 5, 6]
161QPSFEPTKNKELTDEubiquitination[1, 3, 4]
163SFEPTKNKELTDEFRubiquitination[1, 3, 4]
285AKPNCFRKDPDINMHubiquitination[3, 4]
308ILSVELGKQKKKYMPubiquitination[1, 3]
310SVELGKQKKKYMPYNubiquitination[3]
455MPRHNYHKVAPLVQSubiquitination[1]
466LVQSLCAKHGIEYQSubiquitination[7]
474HGIEYQSKPLLSAFAubiquitination[1, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Isoform 1: Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the desaturation of dihomo-gamma-linoleic acid (DHGLA) (20:3n-6) and eicosatetraenoic acid (20:4n-3) to generate arachidonic acid (AA) (20:4n-6) and eicosapentaenoic acid (EPA)(20:5n-3) respectively. 
Sequence Annotation
 DOMAIN 74 151 Cytochrome b5 heme-binding.
 MOTIF 236 240 Histidine box-1.
 MOTIF 273 277 Histidine box-2.
 MOTIF 439 443 Histidine box-3.  
Keyword
 Alternative splicing; Complete proteome; Electron transport; Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Membrane; Mitochondrion; Oxidoreductase; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 501 AA 
Protein Sequence
MGTRAARPAG LPCGAENPAR RRLALGARQQ IHSWSPRTPS TRLTAPAGPA RGVARPAMAP 60
DPVAAETAAQ GPTPRYFTWD EVAQRSGCEE RWLVIDRKVY NISEFTRRHP GGSRVISHYA 120
GQDATDPFVA FHINKGLVKK YMNSLLIGEL SPEQPSFEPT KNKELTDEFR ELRATVERMG 180
LMKANHVFFL LYLLHILLLD GAAWLTLWVF GTSFLPFLLC AVLLSAVQAQ AGWLQHDFGH 240
LSVFSTSKWN HLLHHFVIGH LKGAPASWWN HMHFQHHAKP NCFRKDPDIN MHPFFFALGK 300
ILSVELGKQK KKYMPYNHQH KYFFLIGPPA LLPLYFQWYI FYFVIQRKKW VDLAWMITFY 360
VRFFLTYVPL LGLKAFLGLF FIVRFLESNW FVWVTQMNHI PMHIDHDRNM DWVSTQLQAT 420
CNVHKSAFND WFSGHLNFQI EHHLFPTMPR HNYHKVAPLV QSLCAKHGIE YQSKPLLSAF 480
ADIIHSLKES GQLWLDAYLH Q 501 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0000248; F:C-5 sterol desaturase activity; TAS:ProtInc.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0007568; P:aging; IEA:Compara.
 GO:0036109; P:alpha-linolenic acid metabolic process; TAS:Reactome.
 GO:0019369; P:arachidonic acid metabolic process; IEA:Compara.
 GO:0007267; P:cell-cell signaling; NAS:UniProtKB.
 GO:0009267; P:cellular response to starvation; IDA:UniProtKB.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0046456; P:icosanoid biosynthetic process; TAS:UniProtKB.
 GO:0043651; P:linoleic acid metabolic process; TAS:Reactome.
 GO:0008654; P:phospholipid biosynthetic process; TAS:UniProtKB.
 GO:0045595; P:regulation of cell differentiation; NAS:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB.
 GO:0032868; P:response to insulin stimulus; IEA:Compara.
 GO:0014070; P:response to organic cyclic compound; IEA:Compara.
 GO:0009744; P:response to sucrose stimulus; IEA:Compara.
 GO:0033189; P:response to vitamin A; IEA:Compara.
 GO:0006636; P:unsaturated fatty acid biosynthetic process; IDA:UniProtKB. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd.
 IPR012171; Fatty_acid/sphinglp_desaturase.
 IPR005804; Fatty_acid_desaturase-1. 
Pfam
 PF00173; Cyt-b5
 PF00487; FA_desaturase 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS
 PR00363; CYTOCHROMEB5.