UniProt Accession

 MARE1_HUMAN; Q15691; B2R6I7; E1P5M8; Q3KQS8 

Genbank Protein ID

 U24166; AK312590; AL035071; CH471077; CH471077; BC106068; BC109281 

Genbank Nucleotide ID

 AAC09471.1; BAG35484.1; CAB53072.1; EAW76348.1; EAW76349.1; AAI06069.1; AAI09282.1 

Protein Name

 Microtubule-associated protein RP/EB family member 1 

Protein Synonyms/Alias

 APC-binding protein EB1; End-binding protein 1; EB1 

Gene Name

 MAPRE1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
66	KKVKFQAKLEHEYIQ	acetylation	[1, 2]
66	KKVKFQAKLEHEYIQ	ubiquitination	[3, 4]
76	HEYIQNFKILQAGFK	acetylation	[2, 5]
76	HEYIQNFKILQAGFK	ubiquitination	[3, 4]
83	KILQAGFKRMGVDKI	acetylation	[5]
83	KILQAGFKRMGVDKI	ubiquitination	[3, 4, 6, 7]
89	FKRMGVDKIIPVDKL	acetylation	[2, 5]
95	DKIIPVDKLVKGKFQ	ubiquitination	[4, 5]
98	IPVDKLVKGKFQDNF	ubiquitination	[7]
100	VDKLVKGKFQDNFEF	ubiquitination	[3]
113	EFVQWFKKFFDANYD	ubiquitination	[3, 4]
122	FDANYDGKDYDPVAA	ubiquitination	[5, 7]
148	LVAPALNKPKKPLTS	acetylation	[2]
148	LVAPALNKPKKPLTS	ubiquitination	[3, 4, 5, 6, 7]
150	APALNKPKKPLTSSS	ubiquitination	[7]
151	PALNKPKKPLTSSSA	ubiquitination	[4]
174	QRTAAAPKAGPGVVR	ubiquitination	[4, 7]
182	AGPGVVRKNPGVGNG	ubiquitination	[7]
212	LTVEDLEKERDFYFG	ubiquitination	[3]
220	ERDFYFGKLRNIELI	ubiquitination	[4]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. 

Sequence Annotation

 DOMAIN 14 116 CH.
 DOMAIN 185 255 EB1 C-terminal.
 REGION 124 268 Interaction with MTUS2/TIP150.
 REGION 185 268 Interaction with CDK5RAP2.
 REGION 208 268 DCTN1-binding.
 REGION 220 242 APC-binding.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 124 124 Phosphotyrosine.  

Keyword

 3D-structure; Acetylation; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Microtubule; Mitosis; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 268 AA 

Protein Sequence

Gene Ontology

 GO:0031253; C:cell projection membrane; IEA:Compara.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0030981; C:cortical microtubule cytoskeleton; IDA:UniProtKB.
 GO:0005881; C:cytoplasmic microtubule; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0035371; C:microtubule plus end; IEA:Compara.
 GO:0051010; F:microtubule plus-end binding; IDA:UniProtKB.
 GO:0008022; F:protein C-terminus binding; TAS:ProtInc.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0031115; P:negative regulation of microtubule polymerization; IDA:UniProtKB.
 GO:0035372; P:protein localization to microtubule; IDA:UniProtKB. 

Interpro

 IPR001715; CH-domain.
 IPR004953; EB1_C.
 IPR027328; MAPRE. 

Pfam

 PF00307; CH
 PF03271; EB1 

SMART

  

PROSITE

 PS50021; CH
 PS51230; EB1_C 

PRINTS