CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015293
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WASH complex subunit FAM21 
Protein Synonyms/Alias
  
Gene Name
 Fam21 
Gene Synonyms/Alias
 D6Wsu116e; Kiaa0592 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
62SRTHEIKKQVDGLIQacetylation[1]
109EVEEQVLKAEAEKAEubiquitination[2]
280IEESAKSKRPTSFADubiquitination[2]
Reference
 [1] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the WASH complex, a complex present at the surface of endosomes that recruits and activates the Arp2/3 complex to induce actin polymerization. The WASH complex plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting. In the complex, it probably mediates the recruitment of the complex to endosome membranes (By similarity). 
Sequence Annotation
 MOD_RES 157 157 Phosphoserine.
 MOD_RES 159 159 Phosphoserine.
 MOD_RES 388 388 Phosphoserine.
 MOD_RES 533 533 Phosphoserine.
 MOD_RES 613 613 Phosphoserine.
 MOD_RES 614 614 Phosphoserine.
 MOD_RES 723 723 Phosphoserine.
 MOD_RES 747 747 Phosphoserine.
 MOD_RES 793 793 Phosphothreonine.
 MOD_RES 795 795 Phosphothreonine.
 MOD_RES 798 798 Phosphoserine.
 MOD_RES 870 870 Phosphoserine.
 MOD_RES 1049 1049 Phosphoserine.
 MOD_RES 1067 1067 Phosphoserine.
 MOD_RES 1172 1172 Phosphoserine.
 MOD_RES 1173 1173 Phosphoserine.
 MOD_RES 1222 1222 Phosphoserine.
 MOD_RES 1333 1333 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Endosome; Membrane; Phosphoprotein; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1334 AA 
Protein Sequence
MNRTSPDSER PPASEPVWER PWSVEEIRRS SQNWSLAADA GLLQFLQEFS QQTISRTHEI 60
KKQVDGLIQE TKATHCRLHN VFNDFLMLSN TQFIENRVYD EEVEEQVLKA EAEKAEQEKT 120
REQKEIDLIP KVQEAVNYGL QVLDSAFEQL DIKAGNSDSE EDDANERVDL ILEPKDLYID 180
RPLPYLIGSK LFMEQEDVGL GELSSEEGSV GSDRGSIVDS EDEKEEEESD EDFASHSDND 240
QNQHTTQISD EEEDDDGDLF ADSEKEGDDI EDIEESAKSK RPTSFADELA ARIKGDISNQ 300
RKEGQTDGKP QKTVKEKKER RTPADDEEDI LFPPPTLTDE DFSPFGSRGG LFSNGQGLFD 360
DEDESDLFKE APRARPAQAP VSEELPPSPK PGKKIPAGAV SVLLGHPDVS GSTSAPSLKE 420
LQKHGQPTPG KSSHLPTPAG LFDDDDNDND EDDNNFFMPS SSKPSKTDKV KSTAIIFDDD 480
EGDLFKEKAE ALPAASVSQT HESKTRADKT IALPSSKNLK LVSETKTQKG LFSDEEDSED 540
LFSSQSSSKP KSASLPSSQP PTSVSLFGDE DEEDSLFGSA AAKKQTSSLQ PQSQEKAKPS 600
EQPSKKTSAL LFSSDEEDQW NIADSHTKLA SDNKSKGELW DSGATQGQEA KAVKKTNLFE 660
DDDDDEVDLF AIAKDSQKKT QRTSLLFEDD AESGSSLFGL PPTSVPSATT KKESVPKVPL 720
LFSDEEDSEV PSGVKPEDLK VDNARVSPEV GSADVASIAQ KEGLLPASDQ EAGGPSDIFS 780
SSSPLDKGAK GRTRTVLSLF DEDEDKVEDE SSTCAPQDGR EKGLKTDSRP KSTGVFQDEE 840
LLFSHKLQKD NDPDVDLFAG TKKIRSSVPS GGSLFGDDED DDLFSSAKTQ PVVPEKKGTL 900
KKDHPVSLKN QDPLDSTQGS KEKSTWKTEP AQDSSGLTPF KSREPSSRIG KIQANLAINP 960
AALLPTVALQ IPGTKPVSSE LAFPSSEPGR SHILESVPTL PGSVEAGVSF DLPAQADTLH 1020
SANKSRVKVR GKRRPQTRAA RRLAAQESSE AEDVTVDRGP VAQLSSSPVL PNGHQPLLQP 1080
RMASGQTSSE TATAPPWEGG PVLSAADRSF FVKSRPQTGN EADLFDSGDI FPKSRGSQSV 1140
EGAGVMAGEP PSHSSGGRKE KSLAFPDLSE GSSTEDLFQS VKPRAAKNRN PFPLLEDEED 1200
LFADPRGKKN ERKPDSHQDS VSKTHDIFED DIFATEAIKP FPKKREKGRT LEPNLFDDNI 1260
DIFADLTVKP KEKSKKKVAA KSMFDDDTDD IFSSGLQAKA SKPKSQSAEA ASEQRSEHKV 1320
ASIFDDPLNA FGSQ 1334 
Gene Ontology
 GO:0005769; C:early endosome; ISS:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0071203; C:WASH complex; ISS:UniProtKB.
 GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB. 
Interpro
 IPR027308; FAM21. 
Pfam
  
SMART
  
PROSITE
  
PRINTS