CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029093
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Spectrin alpha chain, non-erythrocytic 1 
Protein Synonyms/Alias
  
Gene Name
 SPTAN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
335SATQIQVKREELITNubiquitination[1]
592LKSWVNEKMKTATDEacetylation[2]
637ALEKAGQKLIDVNHYacetylation[3]
824IGVQNLLKKHQALQAubiquitination[4]
825GVQNLLKKHQALQAEubiquitination[1]
990PREVTMKKGDILTLLubiquitination[5]
1057DNQTRITKEAGSVSLubiquitination[3, 5]
1132QVEVLQKKFDDFQKDubiquitination[5]
1187PRDETDSKTASPWKSubiquitination[3, 5, 6]
1193SKTASPWKSARLMVHubiquitination[5]
1252TKEWIEEKNQALNTDubiquitination[1]
1288DLAALGDKVNSLGETubiquitination[1]
1334RADQRKAKLGDSHDLacetylation[3]
1369VSSDELAKDVTGAEAubiquitination[1]
1519IAAGHYAKGDISSRRubiquitination[1, 3, 5]
1580TASDESYKDPTNIQVubiquitination[1]
1651QWQFLVQKSAEKSQKubiquitination[1]
1843SDDNTIGKEEIQQRLubiquitination[1]
2057AAKHVQSKAIEARHAacetylation[2]
2057AAKHVQSKAIEARHAubiquitination[1]
2200ERELELQKEQRRQEEacetylation[3]
2426RALSSEGKPYVTKEEacetylation[2, 3]
2431EGKPYVTKEELYQNLacetylation[3]
2431EGKPYVTKEELYQNLubiquitination[4]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
  
Sequence Annotation
  
Keyword
 Calcium; Complete proteome; Reference proteome; Repeat; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2477 AA 
Protein Sequence
MDPSGVKVLE TAEDIQERRQ QVLDRYHRFK ELSTLRRQKL EDSYRFQFFQ RDAEELEKWI 60
QEKLQIASDE NYKDPTNLQG KLQKHQAFEA EVQANSGAIV KLDETGNLMI SEGHFASETI 120
RTRLMELHRQ WELLLEKMRE KGIKLLQAQK LVQYLRECED VMDWINDKEA IVTSEELGQD 180
LEHVEVLQKK FEEFQTDMAA HEERVNEVNQ FAAKLIQEQH PEEELIKTKQ DEVNAAWQRL 240
KGLALQRQGK LFGAAEVQRF NRDVDETISW IKEKEQLMAS DDFGRDLASV QALLRKHEGL 300
ERDLAALEDK VKALCAEADR LQQSHPLSAT QIQVKREELI TNWEQIRTLA AERHARLNDS 360
YRLQRFLADF RDLTSWVTEM KALINADELA SDVAGAEALL DRHQEHKGEI DAHEDSFKSA 420
DESGQALLAA GHYASDEVRE KLTVLSEERA ALLELWELRR QQYEQCMDLQ LFYRDTEQVD 480
NWMSKQEAFL LNEDLGDSLD SVEALLKKHE DFEKSLSAQE EKITALDEFA TKLIQNNHYA 540
MEDVATRRDA LLSRRNALHE RAMRRRAQLA DSFHLQQFFR DSDELKSWVN EKMKTATDEA 600
YKDPSNLQGK VQKHQAFEAE LSANQSRIDA LEKAGQKLID VNHYAKDEVA ARMNEVISLW 660
KKLLEATELK GIKLREANQQ QQFNRNVEDI ELWLYEVEGH LASDDYGKDL TNVQNLQKKH 720
ALLEADVAAH QDRIDGITIQ ARQFQDAGHF DAENIKKKQE ALVARYEALK EPMVARKQKL 780
ADSLRLQQLF RDVEDEETWI REKEPIAAST NRGKDLIGVQ NLLKKHQALQ AEIAGHEPRI 840
KAVTQKGNAM VEEGHFAAED VKAKLHELNQ KWEALKAKAS QRRQDLEDSL QAQQYFADAN 900
EAESWMREKE PIVGSTDYGK DEDSAEALLK KHEALMSDLS AYGSSIQALR EQAQSCRQQV 960
APTDDETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST NKDWWKVEVN DRQGFVPAAY 1020
VKKLDPAQSA SRENLLEEQG SIALRQEQID NQTRITKEAG SVSLRMKQVE ELYHSLLELG 1080
EKRKGMLEKS CKKFMLFREA NELQQWINEK EAALTSEEVG ADLEQVEVLQ KKFDDFQKDL 1140
KANESRLKDI NKVAEDLESE GLMAEEVQAV QQQEVYGMMP RDETDSKTAS PWKSARLMVH 1200
TVATFNSIKE LNERWRSLQQ LAEERSQLLG SAHEVQRFHR DADETKEWIE EKNQALNTDN 1260
YGHDLASVQA LQRKHEGFER DLAALGDKVN SLGETAERLI QSHPESAEDL QEKCTELNQA 1320
WSSLGKRADQ RKAKLGDSHD LQRFLSDFRD LMSWINGIRG LVSSDELAKD VTGAEALLER 1380
HQEHRTEIDA RAGTFQAFEQ FGQQLLAHGH YASPEIKQKL DILDQERADL EKAWVQRRMM 1440
LDQCLELQLF HRDCEQAENW MAAREAFLNT EDKGDSLDSV EALIKKHEDF DKAINVQEEK 1500
IAALQAFADQ LIAAGHYAKG DISSRRNEVL DRWRRLKAQM IEKRSKLGES QTLQQFSRDV 1560
DEIEAWISEK LQTASDESYK DPTNIQVSLM LSKHQKHQAF EAELHANADR IRGVIDMGNS 1620
LIERGACAGS EDAVKARLAA LADQWQFLVQ KSAEKSQKLK EANKQQNFNT GIKDFDFWLS 1680
EVEALLASED YGKDLASVNN LLKKHQLLEA DISAHEDRLK DLNSQADSLM TSSAFDTSQV 1740
KDKRDTINGR FQKIKSMAAS RRAKLNESHR LHQFFRDMDD EESWIKEKKL LVGSEDYGRD 1800
LTGVQNLRKK HKRLEAELAA HEPAIQGVLD TGKKLSDDNT IGKEEIQQRL AQFVEHWKEL 1860
KQLAAARGQR LEESLEYQQF VANVEEEEAW INEKMTLVAS EDYGDTLAAI QGLLKKHEAF 1920
ETDFTVHKDR VNDVCTNGQD LIKKNNHHEE NISSKMKGLN GKVSDLEKAA AQRKAKLDEN 1980
SAFLQFNWKA DVVESWIGEK ENSLKTDDYG RDLSSVQTLL TKQETFDAGL QAFQQEGIAN 2040
ITALKDQLLA AKHVQSKAIE ARHASLMKRW SQLLANSAAR KKKLLEAQSH FRKVEDLFLT 2100
FAKKASAFNS WFENAEEDLT DPVRCNSLEE IKALREAHDA FRSSLSSAQA DFNQLAELDR 2160
QIKSFRVASN PYTWFTMEAL EETWRNLQKI IKERELELQK EQRRQEENDK LRQEFAQHAN 2220
AFHQWIQETR CQPAGASCMV EESGTLESQL EATKRKHQEI RAMRSQLKKI EDLGAAMEEA 2280
LILDNKYTEH STVGLAQQWD QLDQLGMRMQ HNLEQQIQAR NTTGVTEEAL KEFSMMFKHF 2340
DKDKSGRLNH QEFKSCLRSL GYDLPMVEEG EPDPEFEAIL DTVDPNRDGH VSLQEYMAFM 2400
ISRETENVKS SEEIESAFRA LSSEGKPYVT KEELYQNLTR EQADYCVSHM KPYVDGKGRE 2460
LPTAFDYVEF TRSLFVN 2477 
Gene Ontology
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005509; F:calcium ion binding; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR014837; EF-hand_Ca_insen.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR001452; SH3_domain.
 IPR018159; Spectrin/alpha-actinin.
 IPR013315; Spectrin_alpha_SH3.
 IPR002017; Spectrin_repeat. 
Pfam
 PF13499; EF_hand_5
 PF08726; efhand_Ca_insen
 PF00018; SH3_1
 PF00435; Spectrin 
SMART
 SM00054; EFh
 SM00326; SH3
 SM00150; SPEC 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.
 PR01887; SPECTRNALPHA.