CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019877
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ADP-ribosylation factor GTPase-activating protein 2 
Protein Synonyms/Alias
 ARF GAP 2; GTPase-activating protein ZNF289; Zinc finger protein 289 
Gene Name
 Arfgap2 
Gene Synonyms/Alias
 Zfp289; Znf289 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
15TEIQTIFKRLRAIPTacetylation[1]
356TFASGPPKYKDNPFSubiquitination[2]
358ASGPPKYKDNPFSLGubiquitination[2]
428RQKFAGAKAISSDMFubiquitination[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). May regulate coatomer-mediated protein transport from the Golgi complex to the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi- derived membranes to allow fusion with target membranes (By similarity). 
Sequence Annotation
 DOMAIN 11 127 Arf-GAP.
 ZN_FING 26 49 C4-type.
 REGION 97 520 Required for interaction with coatomer
 MOD_RES 145 145 Phosphoserine.
 MOD_RES 336 336 Phosphoserine.
 MOD_RES 431 431 Phosphoserine (By similarity).
 MOD_RES 432 432 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; ER-Golgi transport; Golgi apparatus; GTPase activation; Membrane; Metal-binding; Phosphoprotein; Protein transport; Reference proteome; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 520 AA 
Protein Sequence
MAASPSKTEI QTIFKRLRAI PTNKACFDCG AKSPSWASIT YGVFLCIDCS GVHRSLGVHL 60
SFIRSTELDS NWSWLQLRCM QVGGNANATA FFRQHGCMAN DANTKYTSRA AQMYREKIRQ 120
LGSAALTRHG TDLWIDSMNS APSHSPEKKD SDFFTEHTQA PAWDTAATDP SGTQQPALPS 180
ESSSLAQPEQ GPNTDLLGTS PQASLELKSS IIGKKKPAAA KKGLGAKKGL GAQKVSNQSF 240
TEIERQAQVA EKLREQQAAD AKKQAEESMV ASMRLAYQEL QIDRKKEEKK LQNLEGKKRE 300
QAERLGMGLV SRSSISHSVL SEMQMIEQET PLSAKSSRSQ LDLFDDVGTF ASGPPKYKDN 360
PFSLGETFGS RWDSDAAWGM DRVEEKEPEV TISSIRPISE RTASRREVET RSSGLESSEA 420
RQKFAGAKAI SSDMFFGREV DSEYEARSRL QQLSGSSAIS SSDLFGNMDG AHGGGTVSLG 480
NVLPTADIAQ FKQGVKSVAG KMAVLANGVM NSLQDRYGSY 520 
Gene Ontology
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0008060; F:ARF GTPase activator activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW. 
Interpro
 IPR001164; ArfGAP. 
Pfam
 PF01412; ArfGap 
SMART
 SM00105; ArfGap 
PROSITE
 PS50115; ARFGAP 
PRINTS
 PR00405; REVINTRACTNG.