CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012981
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 N-acetylglucosamine-1-phosphotransferase subunits alpha/beta 
Protein Synonyms/Alias
 GlcNAc-1-phosphotransferase subunits alpha/beta; Stealth protein GNPTAB; UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta; N-acetylglucosamine-1-phosphotransferase subunit alpha; N-acetylglucosamine-1-phosphotransferase subunit beta 
Gene Name
 GNPTAB 
Gene Synonyms/Alias
 GNPTA; KIAA1208 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MLFKLLQRQTYubiquitination[1, 2]
573FSFAEVAKRGVEGAYubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. 
Sequence Annotation
 REPEAT 438 473 LNR 1.
 REPEAT 505 545 LNR 2.
 DOMAIN 1005 1040 EF-hand.
 METAL 449 449 Calcium (By similarity).
 METAL 464 464 Calcium (By similarity).
 METAL 467 467 Calcium (By similarity).
 METAL 516 516 Calcium (By similarity).
 METAL 531 531 Calcium (By similarity).
 METAL 534 534 Calcium (By similarity).
 CARBOHYD 83 83 N-linked (GlcNAc...) (Potential).
 CARBOHYD 114 114 N-linked (GlcNAc...) (Potential).
 CARBOHYD 148 148 N-linked (GlcNAc...) (Potential).
 CARBOHYD 179 179 N-linked (GlcNAc...) (Potential).
 CARBOHYD 250 250 N-linked (GlcNAc...) (Potential).
 CARBOHYD 614 614 N-linked (GlcNAc...) (Potential).
 CARBOHYD 699 699 N-linked (GlcNAc...).
 CARBOHYD 729 729 N-linked (GlcNAc...) (Potential).
 CARBOHYD 829 829 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1009 1009 N-linked (GlcNAc...) (Potential).
 CARBOHYD 1129 1129 N-linked (GlcNAc...) (Potential).
 DISULFID 438 461 By similarity.
 DISULFID 452 468 By similarity.
 DISULFID 505 528 By similarity.
 DISULFID 519 535 By similarity.  
Keyword
 Alternative splicing; Calcium; Complete proteome; Disease mutation; Disulfide bond; Glycoprotein; Golgi apparatus; Membrane; Metal-binding; Mucolipidosis; Polymorphism; Reference proteome; Repeat; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1256 AA 
Protein Sequence
MLFKLLQRQT YTCLSHRYGL YVCFLGVVVT IVSAFQFGEV VLEWSRDQYH VLFDSYRDNI 60
AGKSFQNRLC LPMPIDVVYT WVNGTDLELL KELQQVREQM EEEQKAMREI LGKNTTEPTK 120
KSEKQLECLL THCIKVPMLV LDPALPANIT LKDLPSLYPS FHSASDIFNV AKPKNPSTNV 180
SVVVFDSTKD VEDAHSGLLK GNSRQTVWRG YLTTDKEVPG LVLMQDLAFL SGFPPTFKET 240
NQLKTKLPEN LSSKVKLLQL YSEASVALLK LNNPKDFQEL NKQTKKNMTI DGKELTISPA 300
YLLWDLSAIS QSKQDEDISA SRFEDNEELR YSLRSIERHA PWVRNIFIVT NGQIPSWLNL 360
DNPRVTIVTH QDVFRNLSHL PTFSSPAIES HIHRIEGLSQ KFIYLNDDVM FGKDVWPDDF 420
YSHSKGQKVY LTWPVPNCAE GCPGSWIKDG YCDKACNNSA CDWDGGDCSG NSGGSRYIAG 480
GGGTGSIGVG QPWQFGGGIN SVSYCNQGCA NSWLADKFCD QACNVLSCGF DAGDCGQDHF 540
HELYKVILLP NQTHYIIPKG ECLPYFSFAE VAKRGVEGAY SDNPIIRHAS IANKWKTIHL 600
IMHSGMNATT IHFNLTFQNT NDEEFKMQIT VEVDTREGPK LNSTAQKGYE NLVSPITLLP 660
EAEILFEDIP KEKRFPKFKR HDVNSTRRAQ EEVKIPLVNI SLLPKDAQLS LNTLDLQLEH 720
GDITLKGYNL SKSALLRSFL MNSQHAKIKN QAIITDETND SLVAPQEKQV HKSILPNSLG 780
VSERLQRLTF PAVSVKVNGH DQGQNPPLDL ETTARFRVET HTQKTIGGNV TKEKPPSLIV 840
PLESQMTKEK KITGKEKENS RMEENAENHI GVTEVLLGRK LQHYTDSYLG FLPWEKKKYF 900
QDLLDEEESL KTQLAYFTDS KNTGRQLKDT FADSLRYVNK ILNSKFGFTS RKVPAHMPHM 960
IDRIVMQELQ DMFPEEFDKT SFHKVRHSED MQFAFSYFYY LMSAVQPLNI SQVFDEVDTD 1020
QSGVLSDREI RTLATRIHEL PLSLQDLTGL EHMLINCSKM LPADITQLNN IPPTQESYYD 1080
PNLPPVTKSL VTNCKPVTDK IHKAYKDKNK YRFEIMGEEE IAFKMIRTNV SHVVGQLDDI 1140
RKNPRKFVCL NDNIDHNHKD AQTVKAVLRD FYESMFPIPS QFELPREYRN RFLHMHELQE 1200
WRAYRDKLKF WTHCVLATLI MFTIFSFFAE QLIALKRKIF PRRRIHKEAS PNRIRV 1256 
Gene Ontology
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003976; F:UDP-N-acetylglucosamine-lysosomal-enzyme N-acetylglucosaminephosphotransferase activity; IEA:EC.
 GO:0046835; P:carbohydrate phosphorylation; IEA:Compara.
 GO:0030154; P:cell differentiation; IEA:InterPro.
 GO:0009306; P:protein secretion; IEA:Compara. 
Interpro
 IPR010506; DMAP1-bd.
 IPR021520; DUF3184.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000800; Notch_dom. 
Pfam
 PF06464; DMAP_binding
 PF11380; DUF3184
 PF00066; Notch 
SMART
 SM00004; NL 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50258; LNR 
PRINTS