CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012765
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Flap endonuclease GEN homolog 1 
Protein Synonyms/Alias
  
Gene Name
 GEN1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14WQILEPVKQHIPLRNubiquitination[1]
99SRYGSSGKSWSQKTGubiquitination[1]
111KTGRSHFKSVLRECLubiquitination[1]
175RNFTMNTKDPHVDCYubiquitination[1]
188CYTMSSIKSKLGLDRubiquitination[1]
219KGVPGVGKEQALKLIubiquitination[1]
224VGKEQALKLIQILKGubiquitination[1]
230LKLIQILKGQSLLQRacetylation[2]
230LKLIQILKGQSLLQRubiquitination[1, 3]
256SPQLLVTKKLAHCSVubiquitination[1]
557PLAIQQIKAVSKSLIubiquitination[1]
681LKERIFTKLSYPQDNubiquitination[1, 4]
696LQPDVNLKTLSILSVubiquitination[1]
704TLSILSVKESCIANSubiquitination[1]
721DCTSHLSKDLPGIPLubiquitination[1]
739SRDSKILKGDQLLQEubiquitination[1]
814APVFGKAKYTTQRMKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Endonuclease which resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. 
Sequence Annotation
 REGION 1 96 N-domain.
 REGION 122 212 I-domain.
 METAL 30 30 Magnesium 1 (By similarity).
 METAL 75 75 Magnesium 1 (By similarity).
 METAL 134 134 Magnesium 1 (By similarity).
 METAL 136 136 Magnesium 1 (By similarity).
 METAL 155 155 Magnesium 2 (By similarity).
 METAL 157 157 Magnesium 2 (By similarity).
 METAL 208 208 Magnesium 2 (By similarity).  
Keyword
 Complete proteome; DNA damage; DNA repair; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 908 AA 
Protein Sequence
MGVNDLWQIL EPVKQHIPLR NLGGKTIAVD LSLWVCEAQT VKKMMGSVMK PHLRNLFFRI 60
SYLTQMDVKL VFVMEGEPPK LKADVISKRN QSRYGSSGKS WSQKTGRSHF KSVLRECLHM 120
LECLGIPWVQ AAGEAEAMCA YLNAGGHVDG CLTNDGDTFL YGAQTVYRNF TMNTKDPHVD 180
CYTMSSIKSK LGLDRDALVG LAILLGCDYL PKGVPGVGKE QALKLIQILK GQSLLQRFNR 240
WNETSCNSSP QLLVTKKLAH CSVCSHPGSP KDHERNGCRL CKSDKYCEPH DYEYCCPCEW 300
HRTEHDRQLS EVENNIKKKA CCCEGFPFHE VIQEFLLNKD KLVKVIRYQR PDLLLFQRFT 360
LEKMEWPNHY ACEKLLVLLT HYDMIERKLG SRNSNQLQPI RIVKTRIRNG VHCFEIEWEK 420
PEHYAMEDKQ HGEFALLTIE EESLFEAAYP EIVAVYQKQK LEIKGKKQKR IKPKENNLPE 480
PDEVMSFQSH MTLKPTCEIF HKQNSKLNSG ISPDPTLPQE SISASLNSLL LPKNTPCLNA 540
QEQFMSSLRP LAIQQIKAVS KSLISESSQP NTSSHNISVI ADLHLSTIDW EGTSFSNSPA 600
IQRNTFSHDL KSEVESELSA IPDGFENIPE QLSCESERYT ANIKKVLDED SDGISPEEHL 660
LSGITDLCLQ DLPLKERIFT KLSYPQDNLQ PDVNLKTLSI LSVKESCIAN SGSDCTSHLS 720
KDLPGIPLQN ESRDSKILKG DQLLQEDYKV NTSVPYSVSN TVVKTCNVRP PNTALDHSRK 780
VDMQTTRKIL MKKSVCLDRH SSDEQSAPVF GKAKYTTQRM KHSSQKHNSS HFKESGHNKL 840
SSPKIHIKET EQCVRSYETA ENEESCFPDS TKSSLSSLQC HKKENNSGTC LDSPLPLRQR 900
LKLRFQST 908 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. 
Interpro
 IPR020045; 5-3_exonuclease_C.
 IPR008918; HhH2.
 IPR006086; XPG-I_dom.
 IPR006084; XPG/Rad2.
 IPR006085; XPG_DNA_repair_N. 
Pfam
 PF00867; XPG_I
 PF00752; XPG_N 
SMART
 SM00279; HhH2
 SM00484; XPGI
 SM00485; XPGN 
PROSITE
 PS00841; XPG_1
 PS00842; XPG_2 
PRINTS
 PR00853; XPGRADSUPER.