| Tag | Content |
|---|
| CPLM ID | CPLM-005053 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 1,3-beta-glucanosyltransferase GAS1 |
Protein Synonyms/Alias | Glycolipid-anchored surface protein 1; Glycoprotein GP115 |
Gene Name | GAS1 |
Gene Synonyms/Alias | GGP1; YMR307W; YM9952.09 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 189 | VRQYISDKNYRKIPV | acetylation | [1] | | 193 | ISDKNYRKIPVGYSS | ubiquitination | [2] | | 240 | WCGKSDFKTSGYADR | acetylation | [1] | | 240 | WCGKSDFKTSGYADR | ubiquitination | [2] | | 337 | SPTSANTKSYSATTS | ubiquitination | [2] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non- reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3- beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis. |
Sequence Annotation | REGION 119 127 Donor substrate binding (By similarity).
ACT_SITE 161 161 Proton donor.
ACT_SITE 262 262 Nucleophile.
BINDING 92 92 Donor substrate; via carbonyl oxygen (By
BINDING 160 160 Donor substrate (By similarity).
BINDING 161 161 Acceptor substrate (By similarity).
BINDING 202 202 Acceptor substrate; via carbonyl oxygen
BINDING 207 207 Acceptor substrate (By similarity).
BINDING 294 294 Donor substrate (By similarity).
LIPID 528 528 GPI-anchor amidated asparagine.
CARBOHYD 40 40 N-linked (GlcNAc...) (Potential).
CARBOHYD 57 57 N-linked (GlcNAc...) (Potential).
CARBOHYD 95 95 N-linked (GlcNAc...) (Potential).
CARBOHYD 149 149 N-linked (GlcNAc...) (Potential).
CARBOHYD 165 165 N-linked (GlcNAc...) (Potential).
CARBOHYD 253 253 N-linked (GlcNAc...) (Potential).
CARBOHYD 283 283 N-linked (GlcNAc...) (Potential).
CARBOHYD 321 321 N-linked (GlcNAc...) (Potential).
CARBOHYD 409 409 N-linked (GlcNAc...) (Potential).
CARBOHYD 495 495 N-linked (GlcNAc...) (Potential).
DISULFID 74 103 By similarity.
DISULFID 216 348 By similarity.
DISULFID 234 265 By similarity.
DISULFID 370 421 By similarity.
DISULFID 372 462 By similarity.
DISULFID 379 445 By similarity.
DISULFID 398 403 By similarity. |
Keyword | Cell membrane; Cell wall; Cell wall biogenesis/degradation; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Reference proteome; Secreted; Signal; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 559 AA |
Protein Sequence | MLFKSLSKLA TAAAFFAGVA TADDVPAIEV VGNKFFYSNN GSQFYIRGVA YQADTANETS 60
GSTVNDPLAN YESCSRDIPY LKKLNTNVIR VYAINTTLDH SECMKALNDA DIYVIADLAA 120
PATSINRDDP TWTVDLFNSY KTVVDTFANY TNVLGFFAGN EVTNNYTNTD ASAFVKAAIR 180
DVRQYISDKN YRKIPVGYSS NDDEDTRVKM TDYFACGDDD VKADFYGINM YEWCGKSDFK 240
TSGYADRTAE FKNLSIPVFF SEYGCNEVTP RLFTEVEALY GSNMTDVWSG GIVYMYFEET 300
NKYGLVSIDG NDVKTLDDFN NYSSEINKIS PTSANTKSYS ATTSDVACPA TGKYWSAATE 360
LPPTPNGGLC SCMNAANSCV VSDDVDSDDY ETLFNWICNE VDCSGISANG TAGKYGAYSF 420
CTPKEQLSFV MNLYYEKSGG SKSDCSFSGS ATLQTATTQA SCSSALKEIG SMGTNSASGS 480
VDLGSGTESS TASSNASGSS SKSNSGSSGS SSSSSSSSAS SSSSSKKNAA TNVKANLAQV 540
VFTSIISLSI AAGVGFALV 559 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |