CPLM-023564

Genbank Nucleotide ID

Maleylacetoacetate isomerase

Protein Synonyms/Alias

MAAI; GSTZ1-1; Glutathione S-transferase zeta 1

Mus musculus (Mouse)

Position	Peptide	Type	References
57	FQTLNPMKQVPALKI	acetylation	[1]
57	FQTLNPMKQVPALKI	succinylation	[1]
95	LLPQDPQKRAIVRMI	ubiquitination	[2]
133	NQMQWAQKVITSGFN	acetylation	[3]
144	SGFNALEKILQSTAG	acetylation	[3]
144	SGFNALEKILQSTAG	ubiquitination	[2]
177	VANAERFKVDLSPYP	acetylation	[1, 3, 4, 5, 6, 7, 8]
177	VANAERFKVDLSPYP	succinylation	[1]
177	VANAERFKVDLSPYP	ubiquitination	[2]
191	PTISHINKELLALEV	acetylation	[5, 8]

[1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[4] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[6] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
[7] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[8] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]

Functional Description

Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7- chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid (By similarity).

DOMAIN 4 87 GST N-terminal.
DOMAIN 92 212 GST C-terminal.
REGION 14 19 Glutathione binding.
REGION 71 72 Glutathione binding.
REGION 115 117 Glutathione binding.
BINDING 45 45 Glutathione.
BINDING 59 59 Glutathione; via amide nitrogen and
BINDING 111 111 Glutathione.

3D-structure; Complete proteome; Cytoplasm; Isomerase; Multifunctional enzyme; Phenylalanine catabolism; Reference proteome; Transferase; Tyrosine catabolism.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:UniProtKB.
GO:0004364; F:glutathione transferase activity; TAS:MGI.
GO:0016034; F:maleylacetoacetate isomerase activity; ISA:MGI.
GO:0006749; P:glutathione metabolic process; IEA:Compara.
GO:0006559; P:L-phenylalanine catabolic process; TAS:MGI.
GO:0006572; P:tyrosine catabolic process; TAS:MGI.

IPR010987; Glutathione-S-Trfase_C-like.
IPR004045; Glutathione_S-Trfase_N.
IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
IPR004046; GST_C.
IPR005955; Mal_ac_isom.
IPR012336; Thioredoxin-like_fold.

PS50405; GST_CTER
PS50404; GST_NTER