CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032212
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Importin subunit beta-1 
Protein Synonyms/Alias
 cDNA, FLJ79320, highly similar to Importin beta-1 subunit 
Gene Name
 KPNB1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46EEPSNNVKLAATNALubiquitination[1]
61LNSLEFTKANFDKESubiquitination[1, 2, 3, 4]
66FTKANFDKESERHFIacetylation[4]
66FTKANFDKESERHFIubiquitination[4]
231PFIKEHIKNPDWRYRacetylation[5]
231PFIKEHIKNPDWRYRubiquitination[1, 2, 3, 4, 6]
392ESLMEIVKNSAKDCYubiquitination[1, 2, 3, 4, 6, 7]
396EIVKNSAKDCYPAVQubiquitination[1, 3, 4, 7]
404DCYPAVQKTTLVIMEubiquitination[1, 3]
449TLQNVLRKVQHQDALubiquitination[1]
506LKYMEAFKPFLGIGLubiquitination[2]
565ENVHRSVKPQILSVFubiquitination[3]
585AIGGEFKKYLEVVLNubiquitination[2, 3, 8]
632TGIVQGLKGDQENVHubiquitination[1, 3, 4]
690AFGKDVLKLVEARPMacetylation[4, 5, 9]
690AFGKDVLKLVEARPMubiquitination[2, 4]
714RSKTNKAKTLATWATubiquitination[1]
722TLATWATKELRKLKNacetylation[4, 5, 9]
722TLATWATKELRKLKNubiquitination[1, 2]
726WATKELRKLKNQA**ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 731 AA 
Protein Sequence
MKESTLEAIG YICQDIDPEQ LQDKSNEILT AIIQGMRKEE PSNNVKLAAT NALLNSLEFT 60
KANFDKESER HFIMQVVCEA TQCPDTRVRV AALQNLVKIM SLYYQYMETY MGPALFAITI 120
EAMKSDIDEV ALQGIEFWSN VCDEEMDLAI EASEAAEQGR PPEHTSKFYA KGALQYLVPI 180
LTQTLTKQDE NDDDDDWNPC KAAGVCLMLL ATCCEDDIVP HVLPFIKEHI KNPDWRYRDA 240
AVMAFGCILE GPEPSQLKPL VIQAMPTLIE LMKDPSVVVR DTAAWTVGRI CELLPEAAIN 300
DVYLAPLLQC LIEGLSAEPR VASNVCWAFS SLAEAAYEAA DVADDQEEPA TYCLSSSFEL 360
IVQKLLETTD RPDGHQNNLR SSAYESLMEI VKNSAKDCYP AVQKTTLVIM ERLQQVLQME 420
SHIQSTSDRI QFNDLQSLLC ATLQNVLRKV QHQDALQISD VVMASLLRMF QSTAGSGGVQ 480
EDALMAVSTL VEVLGGEFLK YMEAFKPFLG IGLKNYAEYQ VCLAAVGLVG DLCRALQSNI 540
IPFCDEVMQL LLENLGNENV HRSVKPQILS VFGDIALAIG GEFKKYLEVV LNTLQQASQA 600
QVDKSDYDMV DYLNELRESC LEAYTGIVQG LKGDQENVHP DVMLVQPRVE FILSFIDHIA 660
GDEDHTDGVV ACAAGLIGDL CTAFGKDVLK LVEARPMIHE LLTEGRRSKT NKAKTLATWA 720
TKELRKLKNQ A 731 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0008565; F:protein transporter activity; IEA:Compara.
 GO:0006610; P:ribosomal protein import into nucleus; IEA:Compara. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR021133; HEAT_type_2.
 IPR027140; KPNB1. 
Pfam
  
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS