CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004110
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutaminase kidney isoform, mitochondrial 
Protein Synonyms/Alias
 GLS; K-glutaminase; L-glutamine amidohydrolase; Glutaminase kidney isoform 68 kDa chain; Glutaminase kidney isoform 65 kDa chain 
Gene Name
 Gls 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
621CKVNPFPKDRWNNTPacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. 
Sequence Annotation
 REPEAT 590 619 ANK 1.
 REPEAT 624 653 ANK 2.
 BINDING 291 291 Substrate (By similarity).
 BINDING 340 340 Substrate (By similarity).
 BINDING 386 386 Substrate (By similarity).
 BINDING 393 393 Substrate (By similarity).
 BINDING 419 419 Substrate (By similarity).
 BINDING 471 471 Substrate (By similarity).
 BINDING 489 489 Substrate; via amide nitrogen (By
 MOD_RES 316 316 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Mitochondrion; Reference proteome; Repeat; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 674 AA 
Protein Sequence
MMRLRGSAML RELLLRPPAA VGGVLRRTQP LGTLCRRPRG GSRPAAGLVA AARLHPWWGG 60
GGRAKGPGSG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP GASPPAAPGP KDSPGETDAF 120
GNSEGKEMVA AGDNKVKQGL LPSLEDLLFY TIAEGQEKIP VHKFITALKS TGLRTSDPRL 180
KECMDMLRLT LQTTSDGVML DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY 240
ESAKKQSGGK VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI 300
AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI KQGVNNAEKF 360
DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL KEKKCFPEGT DMVGILDFYF 420
QLCSIEVTCE SASVMAATLA NGGFCPITGE RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH 480
VGLPAKSGVA GGILLVVPNV MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH 540
FAKKLDPRRE GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA 600
EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ YTPQGDSDDG 660
KENQTVHKNL DGLL 674 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0004359; F:glutaminase activity; ISS:UniProtKB.
 GO:0006537; P:glutamate biosynthetic process; ISS:UniProtKB.
 GO:0006543; P:glutamine catabolic process; ISS:UniProtKB.
 GO:0051289; P:protein homotetramerization; ISS:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR012338; Beta-lactam/transpept-like.
 IPR015868; Glutaminase. 
Pfam
 PF00023; Ank
 PF04960; Glutaminase 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS