UniProt Accession

 END3_ECOLI; P0AB83; P20625 

Genbank Protein ID

 J02857; U00096; AP009048 

Genbank Nucleotide ID

 AAA24227.1; AAC74705.1; BAA15387.1 

Protein Name

 Endonuclease III 

Protein Synonyms/Alias

 DNA-(apurinic or apyrimidinic site) lyase 

Gene Name

 nth 

Gene Synonyms/Alias

 b1633; JW1625 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
77	EGVKTYIKTIGLYNS	acetylation	[1]
154	RTQFAPGKNVEQVEE	acetylation	[1]
162	NVEQVEEKLLKVVPA	acetylation	[1]
165	QVEEKLLKVVPAEFK	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N- glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. 

Sequence Annotation

 DOMAIN 108 127 HhH.
 METAL 187 187 Iron-sulfur (4Fe-4S).
 METAL 194 194 Iron-sulfur (4Fe-4S).
 METAL 197 197 Iron-sulfur (4Fe-4S).
 METAL 203 203 Iron-sulfur (4Fe-4S).  

Keyword

 3D-structure; 4Fe-4S; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Glycosidase; Hydrolase; Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 211 AA 

Protein Sequence

Gene Ontology

 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003906; F:DNA-(apurinic or apyrimidinic site) lyase activity; IEA:EC.
 GO:0004519; F:endonuclease activity; IEA:InterPro.
 GO:0016798; F:hydrolase activity, acting on glycosyl bonds; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006284; P:base-excision repair; IEA:InterPro. 

Interpro

 IPR011257; DNA_glycosylase.
 IPR004036; Endonuclease-III_CS2.
 IPR005759; Endonuclease-III_Nth.
 IPR004035; Endouclease-III_FeS-bd_BS.
 IPR003651; Endouclease3_FeS-loop_motif.
 IPR003265; HhH-GPD_domain.
 IPR000445; HhH_motif.
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR023170; HTH_base_excis_C. 

Pfam

 PF10576; EndIII_4Fe-2S
 PF00633; HHH
 PF00730; HhH-GPD 

SMART

 SM00478; ENDO3c
 SM00525; FES
 SM00278; HhH1 

PROSITE

 PS00764; ENDONUCLEASE_III_1
 PS01155; ENDONUCLEASE_III_2 

PRINTS