CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014787
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytospin-A 
Protein Synonyms/Alias
 Renal carcinoma antigen NY-REN-22; Sperm antigen with calponin homology and coiled-coil domains 1-like; SPECC1-like protein 
Gene Name
 SPECC1L 
Gene Synonyms/Alias
 CYTSA; KIAA0376 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
382PSIERSRKGSSGNASubiquitination[1]
687ETERSDMKETIFELEubiquitination[1]
842SEPTPTVKTLIKSFDubiquitination[2]
846PTVKTLIKSFDSASQubiquitination[1, 2]
896SGPISTSKPLTALSDubiquitination[2]
904PLTALSDKRPNYGEIubiquitination[2]
939VPAMESAKTLSVSRRubiquitination[1, 2, 3]
999SRIREERKDPLSALAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. 
Sequence Annotation
 DOMAIN 1011 1113 CH.
 MOD_RES 54 54 Phosphoserine (By similarity).
 MOD_RES 868 868 Phosphoserine.
 MOD_RES 881 881 Phosphoserine.
 MOD_RES 887 887 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Cell cycle; Cell division; Cell junction; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Gap junction; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1117 AA 
Protein Sequence
MKKASRSVGS VPKVSAISKT QTAEKIKPEN SSSASTGGKL VKPGTAASLS KTKSSDDLLA 60
GMAGGVTVTN GVKGKKSTCP SAAPSASAPA MTTVENKSKI STGTASSTKR STSTGNKESS 120
STRERLRERT RLNQSKKLPS AGQGANDMAL AKRSRSRTAT ECDVRMSKSK SDNQISDRAA 180
LEAKVKDLLT LAKTKDVEIL HLRNELRDMR AQLGINEDHS EGDEKSEKET IMAHQPTDVE 240
STLLQLQEQN TAIREELNQL KNENRMLKDR LNALGFSLEQ RLDNSEKLFG YQSLSPEITP 300
DNQSDGGGTL TSSVEGSAPG SVEDLLSQDE NTLMDHQHSN SMDNLDSECS EVYQPLTSSD 360
DALDAPSSSE SEGIPSIERS RKGSSGNASE VSVACLTERI HQMEENQHST SEELQATLQE 420
LADLQQITQE LNSENERLGE EKVILMESLC QQSDKLEHFS RQIEYFRSLL DEHHISYVID 480
EDVKSGRYME LEQRYMDLAE NARFEREQLL GVQQHLSNTL KMAEQDNKEA QEMIGALKER 540
SHHMERIIES EQKGKAALAA TLEEYKATVA SDQIEMNRLK AQLENEKQKV AELYSIHNSG 600
DKSDIQDLLE SVRLDKEKAE TLASSLQEDL AHTRNDANRL QDAIAKVEDE YRAFQEEAKK 660
QIEDLNMTLE KLRSDLDEKE TERSDMKETI FELEDEVEQH RAVKLHDNLI ISDLENTVKK 720
LQDQKHDMER EIKTLHRRLR EESAEWRQFQ ADLQTAVVIA NDIKSEAQEE IGDLKRRLHE 780
AQEKNEKLTK ELEEIKSRKQ EEERGRVYNY MNAVERDLAA LRQGMGLSRR SSTSSEPTPT 840
VKTLIKSFDS ASQVPNPAAA AIPRTPLSPS PMKTPPAAAV SPMQRHSISG PISTSKPLTA 900
LSDKRPNYGE IPVQEHLLRT SSASRPASLP RVPAMESAKT LSVSRRSSEE VKRDISAQEG 960
ASPASLMAMG TTSPQLSLSS SPTASVTPTT RSRIREERKD PLSALAREYG GSKRNALLKW 1020
CQKKTEGYQN IDITNFSSSW NDGLAFCALL HTYLPAHIPY QELNSQDKRR NFMLAFQAAE 1080
SVGIKSTLDI NEMVRTERPD WQNVMLYVTA IYKYFET 1117 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005921; C:gap junction; IEA:UniProtKB-SubCell.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW. 
Interpro
 IPR001715; CH-domain. 
Pfam
 PF00307; CH 
SMART
 SM00033; CH 
PROSITE
 PS50021; CH 
PRINTS