CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024391
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable threonine--tRNA ligase 2, cytoplasmic 
Protein Synonyms/Alias
 Threonyl-tRNA synthetase; ThrRS; Threonyl-tRNA synthetase-like protein 2 
Gene Name
 TARSL2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
132KHQPIFIKERLKLFEubiquitination[1]
322KCRILNEKVNTATTTubiquitination[2]
385GISFPDNKMMRDWEKubiquitination[1]
760LVVGEKEKIDNAVNVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
  
Keyword
 Alternative splicing; Aminoacyl-tRNA synthetase; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 802 AA 
Protein Sequence
MAAEALAAEA VASRLERQEE DIRWLWSEVE RLRDEQLNAP YSCQAEGPCL TREVAQLRAE 60
NCDLRHRLCS LRLCLAEERS RQATLESAEL EAAQEAGAQP PPSQSQDKDM KKKKMKESEA 120
DSEVKHQPIF IKERLKLFEI LKKDHQLLLA IYGKKGDTSN IITVRVADGQ TVQGEVWKTT 180
PYQVAAEISQ ELAESTVIAK VNGELWDLDR PLEGDSSLEL LTFDNEEAQA VYWHSSAHIL 240
GEAMELYYGG HLCYGPPIEN GFYYDMFIED RAVSSTELSA LENICKAIIK EKQPFERLEV 300
SKEILLEMFK YNKFKCRILN EKVNTATTTV YRCGPLIDLC KGPHVRHTGK IKTIKIFKNS 360
STYWEGNPEM ETLQRIYGIS FPDNKMMRDW EKFQEEAKNR DHRKIGKEQE LFFFHDLSPG 420
SCFFLPRGAF IYNTLTDFIR EEYHKRDFTE VLSPNMYNSK LWEASGHWQH YSENMFTFEI 480
EKDTFALKPM NCPGHCLMFA HRPRSWREMP IRFADFGVLH RNELSGTLSG LTRVRRFQQD 540
DAHIFCTVEQ IEEEIKGCLQ FLQSVYSTFG FSFQLNLSTR PENFLGEIEM WNEAEKQLQN 600
SLMDFGEPWK MNPGDGAFYG PKIDIKIKDA IGRYHQCATI QLDFQLPIRF NLTYVSKDGD 660
DKKRPVIIHR AILGSVERMI AILSENYGGK WPFWLSPRQV MVIPVGPTCE KYALQVSSEF 720
FEEGFMADVD LDHSCTLNKK IRNAQLAQYN FILVVGEKEK IDNAVNVRTR DNKIHGEILV 780
TSAIDKLKNL RKTRTLNAEE AF 802 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004829; F:threonine-tRNA ligase activity; IEA:EC.
 GO:0006435; P:threonyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR012675; Beta-grasp_dom.
 IPR004095; TGS.
 IPR012676; TGS-like.
 IPR002320; Thr-tRNA-ligase_IIa.
 IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
 IPR012947; tRNA_SAD. 
Pfam
 PF03129; HGTP_anticodon
 PF02824; TGS
 PF00587; tRNA-synt_2b
 PF07973; tRNA_SAD 
SMART
 SM00863; tRNA_SAD 
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01047; TRNASYNTHTHR.