UniProt Accession

 MAOM_MOUSE; Q99KE1; Q3TBM8 

Genbank Protein ID

 AK042289; AK050933; AK050980; AK156403; AK171157; BC004709 

Genbank Nucleotide ID

 BAC31216.1; BAC34467.1; BAC34483.1; BAE33701.1; BAE42281.1; AAH04709.1 

Protein Name

 NAD-dependent malic enzyme, mitochondrial 

Protein Synonyms/Alias

 NAD-ME; Malic enzyme 2 

Gene Name

 Me2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
26	VHLKEKGKPLMLNPR	acetylation	[1]
26	VHLKEKGKPLMLNPR	succinylation	[1]
74	RFHRNLKKMTSPLEK	acetylation	[1]
74	RFHRNLKKMTSPLEK	succinylation	[1]
131	GHIFRRPKGLFISIS	acetylation	[1]
156	NWPENHVKAVVVTDG	acetylation	[1, 2]
224	FYMGLYQKRDRSQLY	acetylation	[1]
224	FYMGLYQKRDRSQLY	succinylation	[1]
240	DLMDEFMKAITDRYG	acetylation	[1]
240	DLMDEFMKAITDRYG	succinylation	[1]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

  

Sequence Annotation

 NP_BIND 311 328 NAD (By similarity).
 ACT_SITE 112 112 Proton donor (By similarity).
 ACT_SITE 183 183 Proton acceptor (By similarity).
 METAL 255 255 Divalent metal cation (By similarity).
 METAL 256 256 Divalent metal cation (By similarity).
 METAL 279 279 Divalent metal cation (By similarity).
 BINDING 165 165 NAD (By similarity).
 BINDING 279 279 NAD (By similarity).
 BINDING 421 421 NAD (By similarity).
 MOD_RES 156 156 N6-acetyllysine (By similarity).
 MOD_RES 224 224 N6-acetyllysine (By similarity).
 MOD_RES 240 240 N6-acetyllysine (By similarity).
 MOD_RES 272 272 N6-acetyllysine (By similarity).
 MOD_RES 346 346 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Allosteric enzyme; Complete proteome; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 589 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0016619; F:malate dehydrogenase (oxaloacetate-decarboxylating) activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:InterPro. 

Interpro

 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR001891; Malic_OxRdtase.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF00390; malic
 PF03949; Malic_M 

SMART

 SM00919; Malic_M 

PROSITE

 PS00331; MALIC_ENZYMES 

PRINTS

 PR00072; MALOXRDTASE.