UniProt Accession

 GTF2I_MOUSE; Q9ESZ8; O54700; O55030; O55031; Q8VHD1; Q8VHD2; Q8VHD3; Q8VHD4; Q9CSB5; Q9D9K9 

Genbank Protein ID

 AF017085; AF043219; AF043220; AF325177; AY030290; AY030291; AY030292; AY030293; BC053044; AK006796; AK013348; AF289666 

Genbank Nucleotide ID

 AAC53569.1; AAC02990.1; AAC02991.1; AAK49785.1; AAK49786.1; AAK49787.1; AAK49788.1; AAH53044.1; BAB24743.1; BAB28803.2; AAF99338.1 

Protein Name

 General transcription factor II-I 

Protein Synonyms/Alias

 GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135 

Gene Name

 Gtf2i 

Gene Synonyms/Alias

 Bap135; Diws1t 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
219	SGSCGPIKVKTEPTE	ubiquitination	[1]
879	VNDLFSRKFGEAIGM	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B- lymphocyte activation. 

Sequence Annotation

 REPEAT 103 197 GTF2I-like 1.
 REPEAT 352 446 GTF2I-like 2.
 REPEAT 457 551 GTF2I-like 3.
 REPEAT 562 656 GTF2I-like 4.
 REPEAT 724 818 GTF2I-like 5.
 REPEAT 859 953 GTF2I-like 6.
 MOTIF 319 326 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 103 103 Phosphoserine (By similarity).
 MOD_RES 207 207 Phosphoserine (By similarity).
 MOD_RES 210 210 Phosphoserine (By similarity).
 MOD_RES 248 248 Phosphotyrosine; by BTK (By similarity).
 MOD_RES 317 317 Phosphotyrosine; by BTK (By similarity).
 MOD_RES 398 398 Phosphotyrosine; by BTK (By similarity).
 MOD_RES 412 412 Phosphoserine; by PKG/PRKG1 (By
 MOD_RES 503 503 Phosphotyrosine; by BTK (By similarity).
 MOD_RES 558 558 Phosphothreonine (By similarity).
 MOD_RES 668 668 Phosphoserine (By similarity).
 MOD_RES 784 784 Phosphoserine; by PKG/PRKG1 (By
 MOD_RES 823 823 Phosphoserine (By similarity).  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 998 AA 

Protein Sequence

Gene Ontology

 GO:0042995; C:cell projection; IDA:MGI.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0051019; F:mitogen-activated protein kinase binding; IDA:MGI.
 GO:0009790; P:embryo development; IMP:MGI.
 GO:0016525; P:negative regulation of angiogenesis; IEA:Compara.
 GO:0051481; P:reduction of cytosolic calcium ion concentration; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004212; GTF2I.
 IPR016659; TF_II-I. 

Pfam

 PF02946; GTF2I 

SMART

  

PROSITE

 PS51139; GTF2I 

PRINTS