CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009366
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 10 kDa heat shock protein, mitochondrial 
Protein Synonyms/Alias
 Hsp10; 10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF 
Gene Name
 HSPE1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
8MAGQAFRKFLPLFDRubiquitination[1, 2, 3]
28SAAETVTKGGIMLPEubiquitination[1, 2, 3, 4, 5]
36GGIMLPEKSQGKVLQubiquitination[2, 5, 6]
40LPEKSQGKVLQATVVacetylation[7, 8]
40LPEKSQGKVLQATVVmalonylation[9]
40LPEKSQGKVLQATVVubiquitination[1, 2, 3]
54VAVGSGSKGKGGEIQmalonylation[9]
56VGSGSKGKGGEIQPVacetylation[10, 11]
56VGSGSKGKGGEIQPVmalonylation[9]
56VGSGSKGKGGEIQPVubiquitination[5]
66EIQPVSVKVGDKVLLacetylation[12]
66EIQPVSVKVGDKVLLubiquitination[1, 2, 3]
70VSVKVGDKVLLPEYGacetylation[10]
70VSVKVGDKVLLPEYGubiquitination[2]
80LPEYGGTKVVLDDKDubiquitination[2]
86TKVVLDDKDYFLFRDacetylation[10]
86TKVVLDDKDYFLFRDubiquitination[1, 2, 3]
99RDGDILGKYVD****acetylation[10]
99RDGDILGKYVD****ubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [8] Generation of acetyllysine antibodies and affinity enrichment of acetylated peptides.
 Guan KL, Yu W, Lin Y, Xiong Y, Zhao S.
 Nat Protoc. 2010 Sep;5(9):1583-95. [PMID: 21085124]
 [9] The first identification of lysine malonylation substrates and its regulatory enzyme.
 Peng C, Lu Z, Xie Z, Cheng Z, Chen Y, Tan M, Luo H, Zhang Y, He W, Yang K, Zwaans BM, Tishkoff D, Ho L, Lombard D, He TC, Dai J, Verdin E, Ye Y, Zhao Y.
 Mol Cell Proteomics. 2011 Dec;10(12):M111.012658. [PMID: 21908771]
 [10] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [11] Aspirin acetylates multiple cellular proteins in HCT-116 colon cancer cells: Identification of novel targets.
 Marimuthu S, Chivukula RS, Alfonso LF, Moridani M, Hagen FK, Bhat GJ.
 Int J Oncol. 2011 Nov;39(5):1273-83. [PMID: 21743961]
 [12] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330
Functional Description
 Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 40 40 N6-malonyllysine.
 MOD_RES 54 54 N6-malonyllysine.
 MOD_RES 56 56 N6-acetyllysine; alternate.
 MOD_RES 56 56 N6-malonyllysine; alternate.
 MOD_RES 79 79 Phosphothreonine.
 MOD_RES 86 86 N6-acetyllysine.
 MOD_RES 99 99 N6-acetyllysine.  
Keyword
 Acetylation; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Phosphoprotein; Reference proteome; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 102 AA 
Protein Sequence
MAGQAFRKFL PLFDRVLVER SAAETVTKGG IMLPEKSQGK VLQATVVAVG SGSKGKGGEI 60
QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY VD 102 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0051087; F:chaperone binding; ISS:UniProtKB.
 GO:0051082; F:unfolded protein binding; NAS:UniProtKB.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; NAS:UniProtKB.
 GO:0006457; P:protein folding; TAS:ProtInc.
 GO:0006986; P:response to unfolded protein; TAS:ProtInc. 
Interpro
 IPR020818; Chaperonin_Cpn10.
 IPR018369; Chaprnonin_Cpn10_CS.
 IPR011032; GroES-like. 
Pfam
 PF00166; Cpn10 
SMART
 SM00883; Cpn10 
PROSITE
 PS00681; CHAPERONINS_CPN10 
PRINTS
 PR00297; CHAPERONIN10.