UniProt Accession

 SRP09_HUMAN; P49458; A8K0N0; Q6NVX0; Q8WTW0 

Genbank Protein ID

 U20998; EF488978; AK289595; CH471066; CH471098; CH471098; BC008443; BC067845; BC021995; BC015094; BC022415; BC066957 

Genbank Nucleotide ID

 AAA70170.1; ABP02070.1; BAF82284.1; EAW50106.1; EAW69750.1; EAW69751.1; AAH08443.1; AAH67845.1; AAH21995.1; AAH15094.1; AAH22415.1; AAH66957.1 

Protein Name

 Signal recognition particle 9 kDa protein 

Protein Synonyms/Alias

 SRP9 

Gene Name

 SRP9 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
16	EFSRAAEKLYLADPM	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
24	LYLADPMKARVVLKY	ubiquitination	[1, 2, 6, 7]
30	MKARVVLKYRHSDGN	acetylation	[8]
30	MKARVVLKYRHSDGN	ubiquitination	[6]
41	SDGNLCVKVTDDLVC	acetylation	[9]
52	DLVCLVYKTDQAQDV	acetylation	[8, 9, 10]
52	DLVCLVYKTDQAQDV	ubiquitination	[4, 6, 9]
60	TDQAQDVKKIEKFHS	acetylation	[9]
60	TDQAQDVKKIEKFHS	ubiquitination	[2, 6]
61	DQAQDVKKIEKFHSQ	ubiquitination	[1, 2, 5, 6, 7]
64	QDVKKIEKFHSQLMR	ubiquitination	[1, 6, 7, 9]
76	LMRLMVAKEARNVTM	ubiquitination	[1, 4, 5, 6, 7, 9]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding. 

Sequence Annotation

 MOD_RES 52 52 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Reference proteome; Ribonucleoprotein; RNA-binding; Signal recognition particle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 86 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005785; C:signal recognition particle receptor complex; TAS:ProtInc.
 GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; NAS:UniProtKB.
 GO:0008312; F:7S RNA binding; IEA:InterPro.
 GO:0003723; F:RNA binding; TAS:ProtInc.
 GO:0005047; F:signal recognition particle binding; TAS:ProtInc.
 GO:0045900; P:negative regulation of translational elongation; IEA:InterPro.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
 GO:0006412; P:translation; TAS:Reactome. 

Interpro

 IPR008832; Signal_recog_particle_SRP9.
 IPR009018; Signal_recog_particle_SRP9/14. 

Pfam

 PF05486; SRP9-21 

SMART

  

PROSITE

  

PRINTS