CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015156
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Enhancer of mRNA-decapping protein 4 
Protein Synonyms/Alias
 Autoantigen Ge-1; Autoantigen RCD-8; Human enhancer of decapping large subunit; Hedls 
Gene Name
 EDC4 
Gene Synonyms/Alias
 HEDLS 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
125VKIQPVAKYDWEQKYacetylation[1, 2]
125VKIQPVAKYDWEQKYubiquitination[3, 4, 5]
287PVDVSQIKQGFIVVKubiquitination[4]
589LSLSSETKPKLMTPDubiquitination[4]
1082TAVEGSMKENISKLLubiquitination[4, 6]
1092ISKLLKSKNLTDAIAubiquitination[4, 6]
1157QQLESHMKSRKAREQubiquitination[4, 7, 8, 9]
1368VMAQVRQKLFQFLQAubiquitination[4]
1383EPHNSLGKAARRLSLubiquitination[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [9] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro). 
Sequence Annotation
 REPEAT 174 214 WD 1.
 REPEAT 230 277 WD 2.
 REPEAT 295 334 WD 3.
 REPEAT 342 393 WD 4.
 REGION 913 934 Sufficient for nuclear localization.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 125 125 N6-acetyllysine.
 MOD_RES 693 693 Phosphothreonine.
 MOD_RES 723 723 Phosphoserine.
 MOD_RES 725 725 Phosphoserine.
 MOD_RES 729 729 Phosphoserine.
 MOD_RES 741 741 Phosphoserine.
 MOD_RES 821 821 Phosphothreonine.
 MOD_RES 844 844 Phosphoserine.
 MOD_RES 871 871 Phosphoserine.
 MOD_RES 875 875 Phosphoserine.
 MOD_RES 879 879 Phosphoserine.
 MOD_RES 887 887 Phosphoserine (By similarity).
 MOD_RES 892 892 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1401 AA 
Protein Sequence
MASCASIDIE DATQHLRDIL KLDRPAGGPS AESPRPSSAY NGDLNGLLVP DPLCSGDSTS 60
ANKTGLRTMP PINLQEKQVI CLSGDDSSTC IGILAKEVEI VASSDSSISS KARGSNKVKI 120
QPVAKYDWEQ KYYYGNLIAV SNSFLAYAIR AANNGSAMVR VISVSTSERT LLKGFTGSVA 180
DLAFAHLNSP QLACLDEAGN LFVWRLALVN GKIQEEILVH IRQPEGTPLN HFRRIIWCPF 240
IPEESEDCCE ESSPTVALLH EDRAEVWDLD MLRSSHSTWP VDVSQIKQGF IVVKGHSTCL 300
SEGALSPDGT VLATASHDGY VKFWQIYIEG QDEPRCLHEW KPHDGRPLSC LLFCDNHKKQ 360
DPDVPFWRFL ITGADQNREL KMWCTVSWTC LQTIRFSPDI FSSVSVPPSL KVCLDLSAEY 420
LILSDVQRKV LYVMELLQNQ EEGHACFSSI SEFLLTHPVL SFGIQVVSRC RLRHTEVLPA 480
EEENDSLGAD GTHGAGAMES AAGVLIKLFC VHTKALQDVQ IRFQPQLNPD VVAPLPTHTA 540
HEDFTFGESR PELGSEGLGS AAHGSQPDLR RIVELPAPAD FLSLSSETKP KLMTPDAFMT 600
PSASLQQITA SPSSSSSGSS SSSSSSSSSL TAVSAMSSTS AVDPSLTRPP EELTLSPKLQ 660
LDGSLTMSSS GSLQASPRGL LPGLLPAPAD KLTPKGPGQV PTATSALSLE LQEVEPLGLP 720
QASPSRTRSP DVISSASTAL SQDIPEIASE ALSRGFGSSA PEGLEPDSMA SAASALHLLS 780
PRPRPGPELG PQLGLDGGPG DGDRHNTPSL LEAALTQEAS TPDSQVWPTA PDITRETCST 840
LAESPRNGLQ EKHKSLAFHR PPYHLLQQRD SQDASAEQSD HDDEVASLAS ASGGFGTKVP 900
APRLPAKDWK TKGSPRTSPK LKRKSKKDDG DAAMGSRLTE HQVAEPPEDW PALIWQQQRE 960
LAELRHSQEE LLQRLCTQLE GLQSTVTGHV ERALETRHEQ EQRRLERALA EGQQRGGQLQ 1020
EQLTQQLSQA LSSAVAGRLE RSIRDEIKKT VPPCVSRSLE PMAGQLSNSV ATKLTAVEGS 1080
MKENISKLLK SKNLTDAIAR AAADTLQGPM QAAYREAFQS VVLPAFEKSC QAMFQQINDS 1140
FRLGTQEYLQ QLESHMKSRK AREQEAREPV LAQLRGLVST LQSATEQMAA TVAGSVRAEV 1200
QHQLHVAVGS LQESILAQVQ RIVKGEVSVA LKEQQAAVTS SIMQAMRSAA GTPVPSAHLD 1260
CQAQQAHILQ LLQQGHLNQA FQQALTAADL NLVLYVCETV DPAQVFGQPP CPLSQPVLLS 1320
LIQQLASDLG TRTDLKLSYL EEAVMHLDHS DPITRDHMGS VMAQVRQKLF QFLQAEPHNS 1380
LGKAARRLSL MLHGLVTPSL P 1401 
Gene Ontology
 GO:0000932; C:cytoplasmic mRNA processing body; IDA:MGI.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; TAS:Reactome.
 GO:0010467; P:gene expression; TAS:Reactome. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
  
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS