UniProt Accession

 FGR_RAT; Q6P6U0; Q63206 

Genbank Protein ID

 X57018; CH473968; CH473968; CH473968; BC062025 

Genbank Nucleotide ID

 CAA40337.1; EDL80655.1; EDL80656.1; EDL80657.1; AAH62025.1 

Protein Name

 Tyrosine-protein kinase Fgr 

Protein Synonyms/Alias

 Proto-oncogene c-Fgr; p55-Fgr 

Gene Name

 Fgr 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
88	GDDLTFTKGEKFHIL	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCER1G and FCGR2. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2 (By similarity). Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. 

Sequence Annotation

 DOMAIN 65 126 SH3.
 DOMAIN 132 229 SH2.
 DOMAIN 251 504 Protein kinase.
 NP_BIND 257 265 ATP (By similarity).
 ACT_SITE 370 370 Proton acceptor (By similarity).
 BINDING 279 279 ATP (By similarity).
 MOD_RES 25 25 Phosphoserine (By similarity).
 MOD_RES 32 32 Phosphotyrosine (By similarity).
 MOD_RES 196 196 Phosphotyrosine (By similarity).
 MOD_RES 400 400 Phosphotyrosine; by autocatalysis.
 MOD_RES 511 511 Phosphotyrosine; by SRC.
 MOD_RES 517 517 Phosphothreonine (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).
 LIPID 3 3 S-palmitoyl cysteine (By similarity).
 LIPID 6 6 S-palmitoyl cysteine (By similarity).  

Keyword

 ATP-binding; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Immunity; Innate immunity; Kinase; Lipoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Myristate; Nucleotide-binding; Palmitate; Phosphoprotein; Proto-oncogene; Reference proteome; SH3 domain; Transferase; Tyrosine-protein kinase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 517 AA 

Protein Sequence

Gene Ontology

 GO:0015629; C:actin cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0034987; F:immunoglobulin receptor binding; ISS:UniProtKB.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:EC.
 GO:0001784; F:phosphotyrosine binding; ISS:UniProtKB.
 GO:0019901; F:protein kinase binding; ISS:UniProtKB.
 GO:0004713; F:protein tyrosine kinase activity; IDA:RGD.
 GO:0050830; P:defense response to Gram-positive bacterium; ISS:UniProtKB.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0007229; P:integrin-mediated signaling pathway; ISS:UniProtKB.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; ISS:UniProtKB.
 GO:0030335; P:positive regulation of cell migration; ISS:UniProtKB.
 GO:0050715; P:positive regulation of cytokine secretion; ISS:UniProtKB.
 GO:0043306; P:positive regulation of mast cell degranulation; ISS:UniProtKB.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IEA:Compara.
 GO:0014068; P:positive regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0008360; P:regulation of cell shape; ISS:UniProtKB.
 GO:0045088; P:regulation of innate immune response; ISS:UniProtKB.
 GO:0050764; P:regulation of phagocytosis; ISS:UniProtKB.
 GO:0045859; P:regulation of protein kinase activity; ISS:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR000980; SH2.
 IPR001452; SH3_domain.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom. 

Pfam

 PF07714; Pkinase_Tyr
 PF00017; SH2
 PF00018; SH3_1 

SMART

 SM00252; SH2
 SM00326; SH3
 SM00219; TyrKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS50001; SH2
 PS50002; SH3 

PRINTS

 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.
 PR00109; TYRKINASE.