UniProt Accession

 DUS1_YEAST; P53759; D6W0K3 

Genbank Protein ID

 Z46373; BK006946 

Genbank Nucleotide ID

 CAA86498.1; DAA09817.1 

Protein Name

 tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] 

Protein Synonyms/Alias

 tRNA-dihydrouridine synthase 1 

Gene Name

 DUS1 

Gene Synonyms/Alias

 YML080W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
169	RIFDDCEKSLNYAKM	ubiquitination	[1]
391	VNGITGDKRVMQGLI	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U16 and U17 in cytoplasmic tRNAs. 

Sequence Annotation

  

Keyword

 Complete proteome; FAD; Flavoprotein; NAD; NADP; Oxidoreductase; Reference proteome; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 423 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:SGD.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0017150; F:tRNA dihydrouridine synthase activity; IDA:SGD. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR001269; tRNA_hU_synthase.
 IPR018517; tRNA_hU_synthase_CS. 

Pfam

 PF01207; Dus 

SMART

  

PROSITE

 PS01136; UPF0034 

PRINTS