UniProt Accession

 IF4A2_RAT; Q5RKI1 

Genbank Protein ID

 BC085859 

Genbank Nucleotide ID

 AAH85859.1 

Protein Name

 Eukaryotic initiation factor 4A-II 

Protein Synonyms/Alias

 eIF-4A-II; eIF4A-II; ATP-dependent RNA helicase eIF4A-2 

Gene Name

 Eif4a2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
55	IYAYGFEKPSAIQQR	ubiquitination	[1]
175	NRRYLSPKWIKMFVL	acetylation	[2]
194	EMLSRGFKDQIYEIF	acetylation	[2]
227	DVLEVTKKFMRDPIR	acetylation	[2]
239	PIRILVKKEELTLEG	acetylation	[2]
292	KVDWLTEKMHARDFT	acetylation	[2]
310	LHGDMDQKERDVIMR	acetylation	[2]
382	NFVTEEDKRILRDIE	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity). 

Sequence Annotation

 DOMAIN 64 235 Helicase ATP-binding.
 DOMAIN 246 407 Helicase C-terminal.
 NP_BIND 77 84 ATP (By similarity).
 MOTIF 33 61 Q motif.
 MOTIF 183 186 DEAD box.  

Keyword

 ATP-binding; Complete proteome; Direct protein sequencing; Helicase; Hydrolase; Initiation factor; Nucleotide-binding; Protein biosynthesis; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 407 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS