CPLM-015772

Genbank Nucleotide ID

COP9 signalosome complex subunit 6

Protein Synonyms/Alias

SGN6; Signalosome subunit 6; JAB1-containing signalosome subunit 6; MOV34 homolog; Vpr-interacting protein; hVIP

Homo sapiens (Human)

Position	Peptide	Type	References
75	VIGALIGKQEGRNIE	ubiquitination	[1, 2, 3]
102	EEKIIIDKEYYYTKE	ubiquitination	[1, 2, 4, 5]
108	DKEYYYTKEEQFKQV	ubiquitination	[1, 2]
113	YTKEEQFKQVFKELE	ubiquitination	[1, 2, 3]
117	EQFKQVFKELEFLGW	ubiquitination	[1, 4, 5]
153	IESPLFLKLNPMTKH	ubiquitination	[2]
229	IAQHSAIKMLHSRVK	ubiquitination	[2]
236	KMLHSRVKLILEYVK	ubiquitination	[2, 4, 5]
243	KLILEYVKASEAGEV	ubiquitination	[1, 2, 3, 4, 5, 6]
274	LPVLSTDKFKTDFYD	ubiquitination	[2, 7]
276	VLSTDKFKTDFYDQC	ubiquitination	[2]
297	AYLGTITKTCNTMNQ	ubiquitination	[2]
308	TMNQFVNKFNVLYDR	ubiquitination	[1, 2, 4, 5, 7]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Has some glucocorticoid receptor- responsive activity. Stabilizes RFWD2/COP1 through reducing RFWD2 auto-ubiquitination and decelerating RFWD2 turnover rate, hence regulates the ubiquitination of RFWD2 targets.

DOMAIN 39 148 MPN.
REGION 211 327 Interaction with Vpr.

Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Nucleus; Reference proteome; Signalosome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0008180; C:signalosome; IDA:UniProtKB.
GO:0010388; P:cullin deneddylation; IDA:UniProtKB.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR000555; JAB1_Mov34_MPN_PAD1.
IPR024969; Rpn11/EIF3F_C.

PF01398; JAB
PF13012; MitMem_reg