CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019371
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Melanoma-associated antigen D4 
Protein Synonyms/Alias
 MAGE-D4 antigen; MAGE-E1 antigen 
Gene Name
 MAGED4; MAGED4B 
Gene Synonyms/Alias
 KIAA1859; MAGED4A; MAGEE1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
313VVAAAAAKAKMATSIubiquitination[1]
315AAAAAKAKMATSIPEubiquitination[1]
422ERANKLVKYLMIKDYubiquitination[2]
427LVKYLMIKDYKKIPIubiquitination[1]
441IKRADMLKDVIREYDubiquitination[1]
495SSARLLGKTKDTPRLubiquitination[2]
530VLWEALRKMGLRPGVubiquitination[1, 3]
547PFLGDLRKLITDDFVubiquitination[1]
555LITDDFVKQKYLEYKubiquitination[1, 2]
557TDDFVKQKYLEYKKIubiquitination[1]
563QKYLEYKKIPNSNPPubiquitination[1]
605NRDPREWKAHFLEAVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. 
Sequence Annotation
 DOMAIN 413 611 MAGE.  
Keyword
 Alternative splicing; Complete proteome; Reference proteome; Tumor antigen; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 741 AA 
Protein Sequence
MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS FDIHILRAFG 60
SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET AARAANVARA AASNRAARAA 120
AAAARTAFSQ VVASHRVATP QVSGEDTQPT TYAAEAQGPT PEPPLASPQT SQMLVTSKMA 180
APEAPATSAQ SQTGSPAQEA ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ 240
PAGVSGMAFP RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW 300
VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK HLDDEYESSE 360
EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR HVLCLPPRNV TLLQERANKL 420
VKYLMIKDYK KIPIKRADML KDVIREYDEH FPEIIERATY TLEKKFGIHL KEIDKEEHLY 480
ILVCTRDSSA RLLGKTKDTP RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP 540
FLGDLRKLIT DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD 600
PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD DIQVELLTWD 660
EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV SSGTNGGAST SVLDGPSTSS 720
TIRTRNAARA GASFFSWIQH R 741 
Gene Ontology
  
Interpro
 IPR002190; MAGE. 
Pfam
 PF01454; MAGE 
SMART
  
PROSITE
 PS50838; MAGE 
PRINTS