UniProt Accession

 HNS_ECOLI; P0ACF8; P08936 

Genbank Protein ID

 X07688; X59940; X57231; X67326; U00096; AP009048 

Genbank Nucleotide ID

 CAA30530.1; CAA42565.1; CAA40507.1; CAA47740.1; AAC74319.1; BAA36117.1 

Protein Name

 DNA-binding protein H-NS 

Protein Synonyms/Alias

 Histone-like protein HLP-II; Protein B1; Protein H1 

Gene Name

 hns 

Gene Synonyms/Alias

 bglY; cur; drdX; hnsA; msyA; osmZ; pilG; topS; b1237; JW1225 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
6	**MSEALKILNNIRT	acetylation	[1]
83	LNSLAAVKSGTKAKR	acetylation	[1]
87	AAVKSGTKAKRAQRP	acetylation	[1]
96	KRAQRPAKYSYVDEN	acetylation	[1]
107	VDENGETKTWTGQGR	acetylation	[1]
120	GRTPAVIKKAMDEQG	acetylation	[1]
121	RTPAVIKKAMDEQGK	acetylation	[1]
128	KAMDEQGKSLDDFLI	acetylation	[1]
136	SLDDFLIKQ******	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 A DNA-binding protein implicated in transcriptional repression (silencing) as well as in bacterial chromosome organization. H-NS binds tightly to AT-rich dsDNA, increases its thermal stability and inhibits transcription. Also binds to ssDNA and RNA but with a much lower affinity. H-NS has possible histone- like function. May be a global transcriptional regulator through its ability to bind to curved DNA sequences, which are found in regions upstream of a certain subset of promoters. Plays a role in the thermal control of pili and adhesive curli fimbriae production, by inducing transcription of csgD. Represses the CRISPR-cas promoters, permits only weak transcription of the crRNA precursor; its role is antagonized by LeuO. Subject to transcriptional auto-repression. Binds preferentially to the upstream region of its own gene recognizing two segments of DNA on both sides of a bend centered around -150. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; DNA-binding; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 137 AA 

Protein Sequence

Gene Ontology

 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0003681; F:bent DNA binding; IDA:EcoliWiki.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR027444; H-NS_C_dom.
 IPR001801; Histone_HNS.
 IPR027454; Histone_HNS_oligo_dom. 

Pfam

 PF00816; Histone_HNS 

SMART

 SM00528; HNS 

PROSITE

  

PRINTS