UniProt Accession

 UBA3_MOUSE; Q8C878; O88598; Q3TG68; Q9D6B7 

Genbank Protein ID

 AK014433; AK032514; AK048148; AK159381; AK168859; CH466523; BC002002; AF077330; AY029181 

Genbank Nucleotide ID

 BAB29346.1; BAC27905.1; BAC33258.1; BAE35036.1; BAE40680.1; EDK99352.1; AAH02002.1; AAC27323.1; AAK33015.1 

Protein Name

 NEDD8-activating enzyme E1 catalytic subunit 

Protein Synonyms/Alias

 NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3 

Gene Name

 Uba3 

Gene Synonyms/Alias

 Ube1c 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
147	NVVPHFNKIQDFNDT	ubiquitination	[1]
260	VRMLQWPKEQPFGDG	ubiquitination	[1]
284	HIQWIFQKSIERASQ	ubiquitination	[1]
307	RLTQGVVKRIIPAVA	ubiquitination	[1]
361	YTFEAERKENCPACS	ubiquitination	[1]
398	NSASLQMKSPAITAT	ubiquitination	[1]
409	ITATLEGKNRTLYLQ	ubiquitination	[1]
434	PNLSKTLKELGLVDG	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. 

Sequence Annotation

 NP_BIND 100 124 ATP (By similarity).
 NP_BIND 148 171 ATP (By similarity).
 REGION 53 70 Interaction with UBE2M N-terminus (By
 REGION 157 161 Interaction with UBE2M N-terminus (By
 REGION 192 217 Interaction with UBE2M N-terminus (By
 REGION 227 229 Interaction with NEDD8 (By similarity).
 REGION 242 248 Interaction with NAE1 (By similarity).
 REGION 292 295 Interaction with NAE1 (By similarity).
 REGION 331 338 Interaction with UBE2M N-terminus (By
 REGION 352 357 Interaction with NEDD8 (By similarity).
 REGION 368 462 Interaction with UBE2M core domain (By
 ACT_SITE 237 237 Glycyl thioester intermediate (By  

Keyword

 Alternative splicing; ATP-binding; Cell cycle; Complete proteome; Ligase; Nucleotide-binding; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 462 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IEA:Compara.
 GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019781; F:NEDD8 activating enzyme activity; TAS:MGI.
 GO:0007113; P:endomitotic cell cycle; IMP:MGI.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0045116; P:protein neddylation; IEA:UniProtKB-UniPathway.
 GO:0051726; P:regulation of cell cycle; IMP:MGI. 

Interpro

 IPR014929; E2_binding.
 IPR009036; Molybdenum_cofac_synth_MoeB.
 IPR016040; NAD(P)-bd_dom.
 IPR000594; ThiF_NAD_FAD-bd.
 IPR023318; Ub_act_enz_dom_a.
 IPR000127; UBact_repeat.
 IPR019572; Ubiquitin-activating_enzyme.
 IPR018074; UBQ-activ_enz_E1_AS. 

Pfam

 PF08825; E2_bind
 PF00899; ThiF
 PF10585; UBA_e1_thiolCys
 PF02134; UBACT 

SMART

  

PROSITE

 PS00536; UBIQUITIN_ACTIVAT_1
 PS00865; UBIQUITIN_ACTIVAT_2 

PRINTS