CPLM-019713

Genbank Nucleotide ID

Protein Synonyms/Alias

C-Myc-binding protein Mm-1; Myc modulator 1

Homo sapiens (Human)

Position	Peptide	Type	References
42	QLKVVQTKYVEAKDC	acetylation	[1]
47	QTKYVEAKDCLNVLN	ubiquitination	[2]
55	DCLNVLNKSNEGKEL	ubiquitination	[2, 3]
60	LNKSNEGKELLVPLT	ubiquitination	[2, 3]
94	GTGYYVEKTAEDAKD	ubiquitination	[4]
112	RKIDFLTKQMEKIQP	acetylation	[1]
112	RKIDFLTKQMEKIQP	ubiquitination	[2, 5, 6]
153	GAAQATAKA******	ubiquitination	[2, 7, 8, 9]

[1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[9] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC.

MOD_RES 2 2 N-acetylalanine.
MOD_RES 42 42 N6-acetyllysine.

Acetylation; Alternative splicing; Chaperone; Complete proteome; Cytoplasm; Nucleus; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; TAS:UniProtKB.
GO:0005634; C:nucleus; TAS:ProtInc.
GO:0016272; C:prefoldin complex; IEA:InterPro.
GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
GO:0051084; P:'de novo' posttranslational protein folding; TAS:Reactome.
GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IMP:BHF-UCL.
GO:0045892; P:negative regulation of transcription, DNA-dependent; IMP:BHF-UCL.
GO:0060041; P:retina development in camera-type eye; IEA:Compara.

IPR011599; PFD_alpha.
IPR027236; PFDN5.
IPR009053; Prefoldin.
IPR004127; Prefoldin_subunit.