CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016941
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 m7GpppN-mRNA hydrolase 
Protein Synonyms/Alias
 Nucleoside diphosphate-linked moiety X motif 20; Nudix motif 20; mRNA-decapping enzyme 2; hDpc 
Gene Name
 DCP2 
Gene Synonyms/Alias
 NUDT20 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
91EKVLDEWKEYKMGVPubiquitination[1]
128KSGWGFPKGKVNKEEubiquitination[1]
130GWGFPKGKVNKEEAPubiquitination[1]
204IEWFSIEKLPCHRNDubiquitination[1]
291SPGDQWVKHRQPLQQubiquitination[1]
416FDHNAIMKILDL***ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. 
Sequence Annotation
 DOMAIN 95 226 Nudix hydrolase.
 MOTIF 129 150 Nudix box.
 METAL 144 144 Manganese (By similarity).
 METAL 148 148 Manganese (By similarity).
 MOD_RES 246 246 Phosphoserine.
 MOD_RES 247 247 Phosphoserine.
 MOD_RES 249 249 Phosphoserine.
 MOD_RES 276 276 Phosphoserine.
 MOD_RES 284 284 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Manganese; Metal-binding; Nonsense-mediated mRNA decay; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 420 AA 
Protein Sequence
METKRVEIPG SVLDDFCSRF ILHIPSEERD NAIRVCFQIE LAHWFYLDFY MQNTPGLPQC 60
GIRDFAKAVF SHCPFLLPQG EDVEKVLDEW KEYKMGVPTY GAIILDETLE NVLLVQGYLA 120
KSGWGFPKGK VNKEEAPHDC AAREVFEETG FDIKDYICKD DYIELRINDQ LARLYIIPGI 180
PKDTKFNPKT RREIRNIEWF SIEKLPCHRN DMTPKSKLGL APNKFFMAIP FIRPLRDWLS 240
RRFGDSSDSD NGFSSTGSTP AKPTVEKLSR TKFRHSQQLF PDGSPGDQWV KHRQPLQQKP 300
YNNHSEMSDL LKGKNQSMRG NGRKQYQDSP NQKKRTNGLQ PAKQQNSLMK CEKKLHPRKL 360
QDNFETDAVY DLPSSSEDQL LEHAEGQPVA CNGHCKFPFS SRAFLSFKFD HNAIMKILDL 420 
Gene Ontology
 GO:0000932; C:cytoplasmic mRNA processing body; IDA:MGI.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0016442; C:RNA-induced silencing complex; IDA:MGI.
 GO:0016896; F:exoribonuclease activity, producing 5'-phosphomonoesters; TAS:Reactome.
 GO:0050072; F:m7G(5')pppN diphosphatase activity; IDA:UniProtKB.
 GO:0030145; F:manganese ion binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0043928; P:exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay; TAS:Reactome.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0071044; P:histone mRNA catabolic process; IMP:UniProtKB.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IMP:UniProtKB. 
Interpro
 IPR007722; mRNA_decapping_BoxA.
 IPR020084; NUDIX_hydrolase_CS.
 IPR000086; NUDIX_hydrolase_dom.
 IPR015797; NUDIX_hydrolase_dom-like. 
Pfam
 PF05026; DCP2
 PF00293; NUDIX 
SMART
  
PROSITE
 PS51462; NUDIX
 PS00893; NUDIX_BOX 
PRINTS