CPLM-008299

Genbank Nucleotide ID

Hsc70-interacting protein

Protein Synonyms/Alias

Hip; Aging-associated protein 2; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; Suppression of tumorigenicity 13 protein

AAG2; FAM10A1; HIP; SNC6

Homo sapiens (Human)

Position	Peptide	Type	References
14	NELRAFVKMCKQDPS	ubiquitination	[1, 2, 3, 4]
17	RAFVKMCKQDPSVLH	ubiquitination	[3]
41	WVESMGGKVPPATQK	ubiquitination	[1, 2, 4, 5]
48	KVPPATQKAKSEENT	ubiquitination	[2]
50	PPATQKAKSEENTKE	ubiquitination	[2]
56	AKSEENTKEEKPDSK	ubiquitination	[2, 5]
59	EENTKEEKPDSKKVE	ubiquitination	[2]
63	KEEKPDSKKVEEDLK	ubiquitination	[2]
118	MDQANDKKVAAIEAL	ubiquitination	[2, 3, 6]
142	DLFTDAIKLNPRLAI	ubiquitination	[1, 3, 4, 6]
153	RLAILYAKRASVFVK	acetylation	[7]
153	RLAILYAKRASVFVK	ubiquitination	[1, 2, 3, 4, 6]
160	KRASVFVKLQKPNAA	ubiquitination	[1, 3, 4, 5, 6]
163	SVFVKLQKPNAAIRD	ubiquitination	[1, 3, 4]
186	PDSAQPYKWRGKAHR	ubiquitination	[1, 2, 3, 4, 5, 6, 8, 9]
222	EDASAMLKEVQPRAQ	ubiquitination	[2]
353	SKYQSNPKVMNLISK	ubiquitination	[2, 3, 5, 6, 9, 10, 11]
360	KVMNLISKLSAKFGG	ubiquitination	[2, 3, 5, 6, 9, 10, 11]
364	LISKLSAKFGGQA**	ubiquitination	[2, 3, 5, 6, 8, 9, 10, 11, 12]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[10] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[11] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[12] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity).

REPEAT 114 147 TPR 1.
REPEAT 148 181 TPR 2.
REPEAT 182 215 TPR 3.
DOMAIN 319 358 STI1.
MOD_RES 346 346 Phosphoserine; by GRK5.

3D-structure; Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; Repeat; TPR repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; TAS:ProtInc.
GO:0005829; C:cytosol; IEA:Compara.
GO:0043234; C:protein complex; IEA:Compara.
GO:0032564; F:dATP binding; IEA:Compara.
GO:0030674; F:protein binding, bridging; TAS:ProtInc.
GO:0070389; P:chaperone cofactor-dependent protein refolding; IEA:Compara.
GO:0061084; P:negative regulation of protein refolding; IEA:Compara.
GO:0006457; P:protein folding; TAS:ProtInc.
GO:0051260; P:protein homooligomerization; IEA:Compara.

IPR006636; STI1_HS-bd.
IPR001440; TPR-1.
IPR013026; TPR-contain_dom.
IPR011990; TPR-like_helical.
IPR013105; TPR_2.
IPR019734; TPR_repeat.

PF00515; TPR_1
PF07719; TPR_2

SM00727; STI1
SM00028; TPR

PS50005; TPR
PS50293; TPR_REGION