CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004500
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Carboxypeptidase E 
Protein Synonyms/Alias
 CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase 
Gene Name
 CPE 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
164LNPDGFEKAASQPGEubiquitination[1]
173ASQPGELKDWFVGRSubiquitination[1]
203RIVYVNEKEGGPNNHubiquitination[1]
224KIVDQNTKLAPETKAubiquitination[1]
406DHDVTSAKDGDYWRLubiquitination[1]
433PGYLAITKKVAVPYSubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. 
Sequence Annotation
 ACT_SITE 342 342 Nucleophile (By similarity).
 METAL 114 114 Zinc (By similarity).
 METAL 117 117 Zinc (By similarity).
 METAL 248 248 Zinc (By similarity).
 CARBOHYD 139 139 N-linked (GlcNAc...) (Potential).
 CARBOHYD 390 390 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Carboxypeptidase; Cleavage on pair of basic residues; Complete proteome; Cytoplasmic vesicle; Glycoprotein; Hydrolase; Membrane; Metal-binding; Metalloprotease; Nucleus; Polymorphism; Protease; Reference proteome; Secreted; Signal; Zinc; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 476 AA 
Protein Sequence
MAGRGGSALL ALCGALAACG WLLGAEAQEP GAPAAGMRRR RRLQQEDGIS FEYHRYPELR 60
EALVSVWLQC TAISRIYTVG RSFEGRELLV IELSDNPGVH EPGEPEFKYI GNMHGNEAVG 120
RELLIFLAQY LCNEYQKGNE TIVNLIHSTR IHIMPSLNPD GFEKAASQPG ELKDWFVGRS 180
NAQGIDLNRN FPDLDRIVYV NEKEGGPNNH LLKNMKKIVD QNTKLAPETK AVIHWIMDIP 240
FVLSANLHGG DLVANYPYDE TRSGSAHEYS SSPDDAIFQS LARAYSSFNP AMSDPNRPPC 300
RKNDDDSSFV DGTTNGGAWY SVPGGMQDFN YLSSNCFEIT VELSCEKFPP EETLKTYWED 360
NKNSLISYLE QIHRGVKGFV RDLQGNPIAN ATISVEGIDH DVTSAKDGDY WRLLIPGNYK 420
LTASAPGYLA ITKKVAVPYS PAAGVDFELE SFSERKEEEK EELMEWWKMM SETLNF 476 
Gene Ontology
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:BHF-UCL.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:ProtInc.
 GO:0030667; C:secretory granule membrane; TAS:Reactome.
 GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0004180; F:carboxypeptidase activity; TAS:ProtInc.
 GO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0003214; P:cardiac left ventricle morphogenesis; IMP:BHF-UCL.
 GO:0006464; P:cellular protein modification process; NAS:UniProtKB.
 GO:0030070; P:insulin processing; IEA:Compara.
 GO:0007218; P:neuropeptide signaling pathway; NAS:UniProtKB.
 GO:0072657; P:protein localization to membrane; IDA:BHF-UCL.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR008969; CarboxyPept-like_regulatory.
 IPR014766; CarboxyPept_regulatory_dom.
 IPR000834; Peptidase_M14. 
Pfam
 PF00246; Peptidase_M14 
SMART
 SM00631; Zn_pept 
PROSITE
 PS00132; CARBOXYPEPT_ZN_1
 PS00133; CARBOXYPEPT_ZN_2 
PRINTS
 PR00765; CRBOXYPTASEA.