UniProt Accession

 ENOB_RAT; P15429; Q5XIV3 

Genbank Protein ID

 Y00979; BC083566; X57774 

Genbank Nucleotide ID

 CAA68788.1; AAH83566.1; CAA40920.1 

Protein Name

 Beta-enolase 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase 

Gene Name

 Eno3 

Gene Synonyms/Alias

 Eno-3 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
5	***MAMQKIFAREIL	acetylation	[1]
28	EVDLHTAKGRFRAAV	acetylation	[1]
54	LELRDGDKSRYLGKG	acetylation	[1]
64	YLGKGVLKAVEHINK	acetylation	[1]
71	KAVEHINKTLGPALL	acetylation	[1]
80	LGPALLEKKLSVVDQ	acetylation	[1]
89	LSVVDQEKVDKFMIE	acetylation	[1]
126	CKAGAAEKGVPLYRH	acetylation	[1]
179	PVGASSFKEAMRIGA	acetylation	[1]
193	AEVYHHLKGVIKAKY	acetylation	[1]
199	LKGVIKAKYGKDATN	acetylation	[1]
202	VIKAKYGKDATNVGD	acetylation	[1]
256	SEFYRNGKYDLDFKS	acetylation	[1]
262	GKYDLDFKSPDDPAR	acetylation	[1]
275	ARHISGEKLGELYKS	acetylation	[1]
281	EKLGELYKSFIKNYP	acetylation	[1]
334	RIAQAVEKKACNCLL	acetylation	[1]
420	IEEALGDKAVFAGRK	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Appears to have a function in striated muscle development and regeneration. 

Sequence Annotation

 REGION 370 373 Substrate binding (By similarity).
 ACT_SITE 210 210 Proton donor (By similarity).
 ACT_SITE 343 343 Proton acceptor (By similarity).
 METAL 245 245 Magnesium (By similarity).
 METAL 293 293 Magnesium (By similarity).
 METAL 318 318 Magnesium (By similarity).
 BINDING 158 158 Substrate (By similarity).
 BINDING 167 167 Substrate (By similarity).
 BINDING 293 293 Substrate (By similarity).
 BINDING 318 318 Substrate (By similarity).
 BINDING 394 394 Substrate (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 434 AA 

Protein Sequence

Gene Ontology

 GO:0016020; C:membrane; IDA:RGD.
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; IDA:RGD.
 GO:0046982; F:protein heterodimerization activity; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0007568; P:aging; IEP:RGD.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0043403; P:skeletal muscle tissue regeneration; IEP:RGD. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.