CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002465
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycolytic genes transcriptional activator GCR1 
Protein Synonyms/Alias
  
Gene Name
 GCR1 
Gene Synonyms/Alias
 SIT3; YPL075W; LPF10W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
619SFPQSSSKFEIINKKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Transcriptional activator required for the expression of glycolytic and ribosomal genes. Forms a transcriptional activation complex with RAP1, RAP1 providing the specific DNA-binding function and GCR1 providing the activation function. Can also bind itself to DNA to a core 5'-CTTCC-3' sequence (CT box). CT box- binding is not essential, but enhances the activation function of the RAP1-GCR1 complex in promoters that contain both DNA signals (only glycolytic genes). CT box-dependent transcriptional activation requires GCR2. 
Sequence Annotation
 REGION 267 299 Leucine-zipper.
 REGION 632 785 DNA-binding.
 MOD_RES 447 447 Phosphothreonine.
 MOD_RES 449 449 Phosphoserine.
 MOD_RES 482 482 Phosphoserine.
 MOD_RES 489 489 Phosphoserine.
 MOD_RES 538 538 Phosphoserine.  
Keyword
 Activator; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 785 AA 
Protein Sequence
MVCTSTSSNF YSIAQYILQS YFKVNVDSLN SLKLVDLIVD QTYPDSLTLR KLNEGATGQP 60
YDYFNTVSRD ADISKCPIFA LTIFFVIRWS HPNPPISIEN FTTVPLLDSN FISLNSNPLL 120
YIQNQNPNSN SSVKVSRSQT FEPSKELIDL VFPWLSYLKQ DMLLIDRTNY KLYSLCELFE 180
FMGRVAIQDL RYLSQHPLLL PNIVTFISKF IPELFQNEEF KGIGSIKNSN NNALNNVTGI 240
ETQFLNPSTE EVSQKVDSYF MELSKKLTTE NIRLSQEITQ LKADMNSVGN VCNQILLLQR 300
QLLSGNQAIG SKSENIVSST GGGILILDKN SINSNVLSNL VQSIDPNHSK PNGQAQTHQR 360
GPKGQSHAQV QSTNSPALAP INMFPSLSNS IQPMLGTLAP QPQDIVQKRK LPLPGSIASA 420
ATGSPFSPSP VGESPYSKRF KLDDKPTPSQ TALDSLLTKS ISSPRLPLST LANTAVTESF 480
RSPQQFQHSP DFVVGGSSSS TTENNSKKVN EDSPSSSSKL AERPRLPNND STTSMPESPT 540
EVAGDDVDRE KPPESSKSEP NDNSPESKDP EKNGKNSNPL GTDADKPVPI SNIHNSTEAA 600
NSSGTVTKTA PSFPQSSSKF EIINKKDTKA GPNEAIKYKL SRENKTIWDL YAEWYIGLNG 660
KSSIKKLIEN YGWRRWKVSE DSHFFPTRRI IMDYIETECD RGIKLGRFTN PQQPREDIRK 720
ILVGDLEKFR INNGLTLNSL SLYFRNLTKN NKEICIFENF KNWNVRSMTE EEKLKYCKRR 780
HNTPS 785 
Gene Ontology
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:SGD.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IDA:SGD.
 GO:0000980; F:RNA polymerase II distal enhancer sequence-specific DNA binding; IDA:SGD.
 GO:0003705; F:RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; IMP:SGD.
 GO:0000433; P:negative regulation of transcription from RNA polymerase II promoter by glucose; IMP:SGD.
 GO:0034728; P:nucleosome organization; IMP:SGD.
 GO:0060963; P:positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0061435; P:positive regulation of transcription from a mobile element promoter; IMP:SGD.
 GO:0072363; P:regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter; IMP:SGD. 
Interpro
 IPR022210; TF_OSM. 
Pfam
 PF12550; GCR1_C 
SMART
  
PROSITE
  
PRINTS