CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002359
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-galactosidase 
Protein Synonyms/Alias
 Melibiase 
Gene Name
 melA 
Gene Synonyms/Alias
 mel-7; b4119; JW4080 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
55ESHIVVRKLMDSAGAacetylation[1]
73ITCHTQQKEALEDADacetylation[1]
Reference
 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
  
Sequence Annotation
 NP_BIND 5 71 NAD (By similarity).
 ACT_SITE 174 174 Proton donor (By similarity).
 METAL 173 173 Manganese (By similarity).
 METAL 203 203 Manganese (By similarity).
 BINDING 151 151 Substrate (By similarity).
 BINDING 287 287 Substrate (By similarity).  
Keyword
 Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glycosidase; Hydrolase; Manganese; Metal-binding; NAD; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 451 AA 
Protein Sequence
MMSAPKITFI GAGSTIFVKN ILGDVFHREA LKTAHIALMD IDPTRLEESH IVVRKLMDSA 60
GASGKITCHT QQKEALEDAD FVVVAFQIGG YEPCTVTDFE VCKRHGLEQT IADTLGPGGI 120
MRALRTIPHL WQICEDMTEV CPDATMLNYV NPMAMNTWAM YARYPHIKQV GLCHSVQGTA 180
EELARDLNID PATLRYRCAG INHMAFYLEL ERKTADGSYV NLYPELLAAY EAGQAPKPNI 240
HGNTRCQNIV RYEMFKKLGY FVTESSEHFA EYTPWFIKPG REDLIERYKV PLDEYPKRCV 300
EQLANWHKEL EEYKKASRID IKPSREYAST IMNAIWTGEP SVIYGNVRND GLIDNLPQGC 360
CVEVACLVDA NGIQPTKVGT LPSHLAALMQ TNINVQTLLT EAILTENRDR VYHAAMMDPH 420
TAAVLGIDEI YALVDDLIAA HGDWLPGWLH R 451 
Gene Ontology
 GO:0005829; C:cytosol; IDA:EcoCyc.
 GO:0004557; F:alpha-galactosidase activity; IDA:EcoCyc.
 GO:0030145; F:manganese ion binding; IDA:EcoCyc.
 GO:0070403; F:NAD+ binding; IDA:EcoCyc.
 GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
 GO:0052692; F:raffinose alpha-galactosidase activity; IEA:EC.
 GO:0005995; P:melibiose catabolic process; IMP:EcoCyc. 
Interpro
 IPR019802; GlycHydrolase_4_CS.
 IPR001088; Glyco_hydro_4.
 IPR022616; Glyco_hydro_4_C.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF02056; Glyco_hydro_4
 PF11975; Glyco_hydro_4C 
SMART
  
PROSITE
 PS01324; GLYCOSYL_HYDROL_F4 
PRINTS
 PR00732; GLHYDRLASE4.