UniProt Accession

 FBX6_HUMAN; Q9NRD1; B1AK42; B2RC88; Q9UKT3 

Genbank Protein ID

 AF129536; AF233223; AK314989; AL031731; AL031731; CH471130; BC020880 

Genbank Nucleotide ID

 AAF04470.1; AAF67153.1; BAG37485.1; CAI20214.1; CAI20215.1; EAW71696.1; AAH20880.1 

Protein Name

 F-box only protein 6 

Protein Synonyms/Alias

 F-box protein that recognizes sugar chains 2; F-box/G-domain protein 2 

Gene Name

 FBXO6 

Gene Synonyms/Alias

 FBG2; FBS2; FBX6 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
66	LREGFITKDWDQPVA	ubiquitination	[1]
260	SSIVVSPKMTRNQAS	ubiquitination	[1]
275	SEAQPGQKHGQEEAA	ubiquitination	[1, 2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Substrate-recognition component of some SCF (SKP1-CUL1- F-box protein)-type E3 ubiquitin ligase complexes. Involved in endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins by recognizing and binding sugar chains on unfolded glycoproteins that are retrotranlocated into the cytosol and promoting their ubiquitination and subsequent degradation. Able to recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex- type oligosaccharides. Also recognizes sulfated glycans. Also involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. Ubiquitination of CHEK1 is required to insure that activated CHEK1 does not accumulate as cells progress through S phase, or when replication forks encounter transient impediments during normal DNA replication. 

Sequence Annotation

 DOMAIN 10 57 F-box.
 DOMAIN 78 259 FBA.  

Keyword

 Complete proteome; Cytoplasm; DNA damage; DNA repair; Polymorphism; Reference proteome; Ubl conjugation pathway; Unfolded protein response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 293 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
 GO:0030246; F:carbohydrate binding; IEA:Compara.
 GO:0001948; F:glycoprotein binding; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
 GO:0000077; P:DNA damage checkpoint; TAS:UniProtKB.
 GO:0006281; P:DNA repair; TAS:UniProtKB.
 GO:0030433; P:ER-associated protein catabolic process; ISS:UniProtKB.
 GO:0006516; P:glycoprotein catabolic process; IEA:Compara.
 GO:0006986; P:response to unfolded protein; IEA:UniProtKB-KW.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB. 

Interpro

 IPR007397; F-box-assoc_dom.
 IPR001810; F-box_dom_cyclin-like.
 IPR008979; Galactose-bd-like. 

Pfam

 PF04300; FBA 

SMART

 SM00256; FBOX 

PROSITE

 PS51114; FBA
 PS50181; FBOX 

PRINTS