CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018864
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin fusion degradation protein 1 homolog 
Protein Synonyms/Alias
 UB fusion protein 1 
Gene Name
 UFD1L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45SDVEKGGKIIMPPSAubiquitination[1]
68ITYPMLFKLTNKNSDubiquitination[1]
72MLFKLTNKNSDRMTHubiquitination[1]
139FLDITNPKAVLENALubiquitination[1]
175ELRVMETKPDKAVSIubiquitination[1, 2]
178VMETKPDKAVSIIECubiquitination[2]
250EPSPSPIKPGDIKRGubiquitination[3]
264GIPNYEFKLGKITFIubiquitination[2, 4]
267NYEFKLGKITFIRNSubiquitination[3, 5]
279RNSRPLVKKVEEDEAubiquitination[1]
303EGQSLRKKGRKP***ubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 231 231 Phosphoserine.
 MOD_RES 245 245 Phosphoserine.
 MOD_RES 247 247 Phosphoserine.
 MOD_RES 299 299 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 307 AA 
Protein Sequence
MFSFNMFDHP IPRVFQNRFS TQYRCFSVSM LAGPNDRSDV EKGGKIIMPP SALDQLSRLN 60
ITYPMLFKLT NKNSDRMTHC GVLEFVADEG ICYLPHWMMQ NLLLEEGGLV QVESVNLQVA 120
TYSKFQPQSP DFLDITNPKA VLENALRNFA CLTTGDVIAI NYNEKIYELR VMETKPDKAV 180
SIIECDMNVD FDAPLGYKEP ERQVQHEEST EGEADHSGYA GELGFRAFSG SGNRLDGKKK 240
GVEPSPSPIK PGDIKRGIPN YEFKLGKITF IRNSRPLVKK VEEDEAGGRF VAFSGEGQSL 300
RKKGRKP 307 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004843; F:ubiquitin-specific protease activity; TAS:ProtInc.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IEA:UniProtKB-UniPathway.
 GO:0001501; P:skeletal system development; TAS:ProtInc.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:ProtInc. 
Interpro
 IPR004854; UFD1. 
Pfam
 PF03152; UFD1 
SMART
  
PROSITE
  
PRINTS