UniProt Accession

 GCN4_YEAST; P03069; D3DLN9; P03068; Q70D88; Q70D91; Q70D96; Q70D99; Q70DA0; Q96UT3 

Genbank Protein ID

 K02205; K02649; AJ585686; AJ585687; AJ585688; AJ585689; AJ585690; AJ585691; AJ585692; AJ585693; AJ585694; AJ585695; AJ585696; AJ585697; AJ585698; AJ585699; AJ585700; AJ585701; AJ585702; AJ585703; AJ585704; AF416613; U18530; BK006939 

Genbank Nucleotide ID

 AAA34640.1; AAA65521.1; CAE52206.1; CAE52207.1; CAE52208.1; CAE52209.1; CAE52210.1; CAE52211.1; CAE52212.1; CAE52213.1; CAE52214.1; CAE52215.1; CAE52216.1; CAE52217.1; CAE52218.1; CAE52219.1; CAE52220.1; CAE52221.1; CAE52222.1; CAE52223.1; CAE52224.1; AAL09032.1; AAB64486.1; DAA07643.1 

Protein Name

 General control protein GCN4 

Protein Synonyms/Alias

 Amino acid biosynthesis regulatory protein 

Gene Name

 GCN4 

Gene Synonyms/Alias

 AAS3; ARG9; YEL009C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
50	LIFDKFIKTEEDPII	sumoylation	[1]
58	TEEDPIIKQDTPSNL	sumoylation	[1]

Reference

 [1] Sumoylation of transcription factor Gcn4 facilitates its Srb10-mediated clearance from promoters in yeast.
 Rosonina E, Duncan SM, Manley JL.
 Genes Dev. 2012 Feb 15;26(4):350-5. [PMID: 22345516] 

Functional Description

 Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA- 3'. 

Sequence Annotation

 DOMAIN 225 281 bZIP.
 REGION 89 100 Required for transcriptional activation.
 REGION 106 125 Required for transcriptional activation.
 REGION 231 251 Basic motif (By similarity).
 REGION 253 274 Leucine-zipper (By similarity).
 MOD_RES 17 17 Phosphoserine.
 MOD_RES 165 165 Phosphothreonine; by PHO85.
 MOD_RES 218 218 Phosphoserine.  

Keyword

 3D-structure; Activator; Amino-acid biosynthesis; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 281 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IDA:SGD.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0001191; F:RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; IPI:SGD.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IMP:SGD.
 GO:0001135; F:RNA polymerase II transcription factor recruiting transcription factor activity; IMP:SGD.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:SGD.
 GO:0001084; F:TFIID-class binding transcription factor activity; IPI:SGD.
 GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
 GO:0010691; P:negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels; IMP:SGD.
 GO:0001080; P:nitrogen catabolite activation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0045899; P:positive regulation of RNA polymerase II transcriptional preinitiation complex assembly; IDA:SGD.
 GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IMP:SGD. 

Interpro

 IPR004827; bZIP. 

Pfam

 PF00170; bZIP_1 

SMART

 SM00338; BRLZ 

PROSITE

 PS50217; BZIP
 PS00036; BZIP_BASIC 

PRINTS