CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030715
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Niemann-Pick C1 protein 
Protein Synonyms/Alias
 cDNA FLJ51802, highly similar to Niemann-Pick C1 protein 
Gene Name
 NPC1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
142SSQARLEKEYFDQHFubiquitination[1, 2, 3]
504SCLFRFFKNSYSPLLubiquitination[1]
692LSDNPNPKCGKGGHAubiquitination[2]
862RAFTVSMKGSRVERAubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 960 AA 
Protein Sequence
MYVIMWITYM AFLLVFFGAF FAVWCYRKRY FVSEYTPIDS NIAFSVNASD KGTAWLLTST 60
FPSSPVLPGE ASCCDPVSAA FEGCLRRLFT RWGSFCVRNP GCVIFFSLVF ITACSSGLVF 120
VRVTTNPVDL WSAPSSQARL EKEYFDQHFG PFFRTEQLII RAPLTDKHIY QPYPSGADVP 180
FGPPLDIQIL HQVLDLQIAI ENITASYDNE TVTLQDICLA PLSPYNTNCT ILSVLNYFQN 240
SHSVLDHKKG DDFFVYADYH THFLYCVRFI NFVKNYKNPN LTISFTAERS IEDELNRESD 300
SDVFTVVISY AIMFLYISLA LGHMKSCRRL LVDSKVSLGI AGILIVLSSV ACSLGVFSYI 360
GLPLTLIVIE VIPFLVLAVG VDNIFILVQA YQRDERLQGE TLDQQLGRVL GEVAPSMFLS 420
SFSETVAFFL GALSVMPAVH TFSLFAGLAV FIDFLLQITC FVSLLGLDIK RQEKNRLDIF 480
CCVRGAEDGT SVQASESCLF RFFKNSYSPL LLKDWMRPIV IAIFVGVLSF SIAVLNKVDI 540
GLDQSLSMPD DSYMVDYFKS ISQYLHAGPP VYFVLEEGHD YTSSKGQNMV CGGMGCNNDS 600
LVQQIFNAAQ LDNYTRIGFA PSSWIDDYFD WVKPQSSCCR VDNITDQFCN ASVVDPACVR 660
CRPLTPEGKQ RPQGGDFMRF LPMFLSDNPN PKCGKGGHAA YSSAVNILLG HGTRVGATYF 720
MTYHTVLQTS ADFIDALKKA RLIASNVTET MGINGSAYRV FPYSVFYVFY EQYLTIIDDT 780
IFNLGVSLGA IFLVTMVLLG CELWSAVIMC ATIAMVLVNM FGVMWLWGIS LNAVSLVNLV 840
MSCGISVEFC SHITRAFTVS MKGSRVERAE EALAHMGSSV FSGITLTKFG GIVVLAFAKS 900
QIFQIFYFRM YLAMVLLGAT HGLIFLPVLL SYIGPSVNKA KSCATEERYK GTERERLLNF 960 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:InterPro.
 GO:0008158; F:hedgehog receptor activity; IEA:InterPro.
 GO:0030301; P:cholesterol transport; IEA:InterPro. 
Interpro
 IPR004765; NP_C_type.
 IPR003392; Patched.
 IPR000731; SSD. 
Pfam
 PF02460; Patched 
SMART
  
PROSITE
 PS50156; SSD 
PRINTS