CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018382
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 1 
Protein Synonyms/Alias
 hBACURD1; BTB/POZ domain-containing protein KCTD13; Polymerase delta-interacting protein 1; TNFAIP1-like protein 
Gene Name
 KCTD13 
Gene Synonyms/Alias
 BACURD1; PDIP1; POLDIP1; FKSG86; PP6832 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20APSLEAPKPSGLEPGubiquitination[1]
34GPAAYGLKPLTPNSKubiquitination[1, 2, 3]
41KPLTPNSKYVKLNVGubiquitination[2, 3]
140CQLALQQKRETLSPLubiquitination[1]
168QLLASTSKPVVKLLHubiquitination[1, 4]
172STSKPVVKLLHNRSNubiquitination[1]
181LHNRSNNKYSYTSTSubiquitination[4]
194TSDDNLLKNIELFDKubiquitination[4]
201KNIELFDKLALRFHGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. 
Sequence Annotation
 DOMAIN 41 109 BTB.  
Keyword
 Complete proteome; Nucleus; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 329 AA 
Protein Sequence
MSAEASGPAA AAAPSLEAPK PSGLEPGPAA YGLKPLTPNS KYVKLNVGGS LHYTTLRTLT 60
GQDTMLKAMF SGRVEVLTDA GGWVLIDRSG RHFGTILNYL RDGSVPLPES TRELGELLGE 120
ARYYLVQGLI EDCQLALQQK RETLSPLCLI PMVTSPREEQ QLLASTSKPV VKLLHNRSNN 180
KYSYTSTSDD NLLKNIELFD KLALRFHGRL LFLKDVLGDE ICCWSFYGQG RKIAEVCCTS 240
IVYATEKKQT KVEFPEARIF EETLNILIYE TPRGPDPALL EATGGAAGAG GAGRGEDEEN 300
REHRVRRIHV RRHITHDERP HGQQIVFKD 329 
Gene Ontology
 GO:0031463; C:Cul3-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0017049; F:GTP-Rho binding; IDA:UniProtKB.
 GO:0016477; P:cell migration; IMP:UniProtKB.
 GO:0006260; P:DNA replication; ISS:UniProtKB.
 GO:0035024; P:negative regulation of Rho protein signal transduction; IMP:UniProtKB.
 GO:0045740; P:positive regulation of DNA replication; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0051260; P:protein homooligomerization; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
 GO:0043149; P:stress fiber assembly; IMP:UniProtKB. 
Interpro
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR003131; T1-type_BTB. 
Pfam
 PF02214; K_tetra 
SMART
 SM00225; BTB 
PROSITE
 PS50097; BTB 
PRINTS