CPLM-012610

Genbank Nucleotide ID

Rho-related GTP-binding protein RhoH

Protein Synonyms/Alias

GTP-binding protein TTF; Translocation three four protein

Homo sapiens (Human)

Position	Peptide	Type	References
6	**MLSSIKCVLVGDS	ubiquitination	[1, 2]
17	VGDSAVGKTSLLVRF	ubiquitination	[2]
97	SFLNLKNKWIGEIRS	ubiquitination	[1, 2]
137	CVNAMEGKKLAQDVR	ubiquitination	[2]
138	VNAMEGKKLAQDVRA	ubiquitination	[2, 3]
146	LAQDVRAKGYLECSA	ubiquitination	[1, 2, 3]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.

NP_BIND 11 18 GTP (By similarity).
NP_BIND 58 62 GTP (By similarity).
NP_BIND 116 119 GTP (By similarity).
REGION 73 86 Interaction with ZAP70 (By similarity).
MOTIF 33 41 Effector region (By similarity).
MOD_RES 188 188 Cysteine methyl ester (By similarity).
LIPID 188 188 S-geranylgeranyl cysteine (By

Cell membrane; Chromosomal rearrangement; Complete proteome; Cytoplasm; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Phosphoprotein; Prenylation; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0001772; C:immunological synapse; IEA:Compara.
GO:0005739; C:mitochondrion; IDA:HPA.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0005525; F:GTP binding; IDA:UniProtKB.
GO:0005095; F:GTPase inhibitor activity; NAS:UniProtKB.
GO:0019210; F:kinase inhibitor activity; IDA:UniProtKB.
GO:0017048; F:Rho GTPase binding; NAS:UniProtKB.
GO:0045576; P:mast cell activation; IEA:Compara.
GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IDA:UniProtKB.
GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
GO:0006355; P:regulation of transcription, DNA-dependent; NAS:UniProtKB.
GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome.
GO:0030217; P:T cell differentiation; NAS:UniProtKB.

IPR027417; P-loop_NTPase.
IPR005225; Small_GTP-bd_dom.
IPR001806; Small_GTPase.
IPR003578; Small_GTPase_Rho.

PR00449; RASTRNSFRMNG.