CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017854
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 THO complex subunit 1 
Protein Synonyms/Alias
 Tho1; Nuclear matrix protein p84 
Gene Name
 Thoc1 
Gene Synonyms/Alias
 Hpr1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
595LAPYLEIKDSDIRQIubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex (By similarity). 
Sequence Annotation
 DOMAIN 570 653 Death.
 MOTIF 414 430 Nuclear localization signal (By
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 Phosphoserine (By similarity).
 MOD_RES 133 133 N6-acetyllysine (By similarity).
 MOD_RES 300 300 N6-acetyllysine (By similarity).
 MOD_RES 560 560 Phosphoserine (By similarity).  
Keyword
 Acetylation; Apoptosis; Complete proteome; Cytoplasm; DNA-binding; mRNA processing; mRNA splicing; mRNA transport; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Transcription; Transcription regulation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 657 AA 
Protein Sequence
MSPTPALFSL PEARTRFTKS TREALNNKNI KPLLTAFSQL PGSENEKKCT LDQAFRGVLE 60
EEIINHSACE NVLAIISLAI GGVTESVCTA STPFVLLGDV LDCLPLDQCD TIFTFVEKNV 120
ATWKSNTFYS AGKNYLLRMC NDLLRRLSKS QNTVFCGRIQ LFLARLFPLS EKSGLNLQSQ 180
FNLENVTVFN TNEQESTLGQ KHTEDREEGM DVEEGEMGDD EAPTTCSIPI DYNLYRKFWS 240
LQDYFRNPVQ CYEKISWKTF LKYSEEVLAV FKSYKLDDTQ ASRKKMEELK TGGEHVYFAK 300
FLTSEKLMDL QLSDSNFRRH ILLQYLILFQ YLKGQVKFKS SNYVLTDEQS LWIEDTTKSV 360
YQLLSENPPD GERFSKMVEH ILNTEENWNS WKNEGCPSFV KERASDTKPT RVVRKRAAPE 420
DFLGKGPNKK ILIGNEELTR LWNLCPDNME ACKSETREYM PTLEEFFEEA IEQADPENMV 480
ESEYKAVNNS NYGWRALRLL ARRSPHFFQP TNQQFKSLPE YLENMVIKLA KELPPPSEEI 540
KTGEDEDEED NDALLKENES PDVRRDKPIT GEQIESFANK LGEQWKILAP YLEIKDSDIR 600
QIECDSEDMK MRAKQLLVAW QDQEGVHATT DNLISALNKS GLSDLAESLT NDTETNS 657 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016363; C:nuclear matrix; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0000445; C:THO complex part of transcription export complex; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0046784; P:intronless viral mRNA export from host nucleus; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0032784; P:regulation of DNA-dependent transcription, elongation; IEA:Compara.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0007165; P:signal transduction; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011029; DEATH-like_dom.
 IPR000488; Death_domain.
 IPR021861; THO_THOC1. 
Pfam
 PF00531; Death
 PF11957; efThoc1 
SMART
 SM00005; DEATH 
PROSITE
 PS50017; DEATH_DOMAIN 
PRINTS