UniProt Accession

 P5CR1_HUMAN; P32322; A6NFM2; Q6FHI4; Q96DI6; Q9HBQ4 

Genbank Protein ID

 M77836; AF218000; CR541769; AC145207; CH471099; BC001504; BC071842 

Genbank Nucleotide ID

 AAA36407.1; AAG17242.1; CAG46568.1; EAW89724.1; AAH01504.1; AAH71842.1 

Protein Name

 Pyrroline-5-carboxylate reductase 1, mitochondrial 

Protein Synonyms/Alias

 P5C reductase 1; P5CR 1 

Gene Name

 PYCR1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
215	QALLGAAKMLLHSEQ	ubiquitination	[1, 2]
228	EQHPGQLKDNVSSPG	ubiquitination	[1]
283	QVSPAAIKKTILDKV	ubiquitination	[1, 2]
291	KTILDKVKLDSPAGT	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. 

Sequence Annotation

 NP_BIND 6 11 NADP.
 NP_BIND 69 72 NADP.
 NP_BIND 95 97 NADP.
 BINDING 34 34 NADP; via carbonyl oxygen.
 BINDING 56 56 NADP.
 MOD_RES 2 2 N-acetylserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Amino-acid biosynthesis; Complete proteome; Direct protein sequencing; Disease mutation; Mitochondrion; NADP; Oxidoreductase; Polymorphism; Proline biosynthesis; Reference proteome; Stress response. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 319 AA 

Protein Sequence

Gene Ontology

 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0004735; F:pyrroline-5-carboxylate reductase activity; IDA:UniProtKB.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0034599; P:cellular response to oxidative stress; IMP:UniProtKB.
 GO:0055129; P:L-proline biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006561; P:proline biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR008927; 6-PGluconate_DH_C-like.
 IPR016040; NAD(P)-bd_dom.
 IPR004455; NADP_OxRdtase_F420.
 IPR000304; Pyrroline-COOH_reductase. 

Pfam

 PF03807; F420_oxidored 

SMART

  

PROSITE

 PS00521; P5CR 

PRINTS