UniProt Accession

 MYL6_MOUSE; Q60605 

Genbank Protein ID

 BC026760; U04443 

Genbank Nucleotide ID

 AAH26760.1; AAC52278.1 

Protein Name

 Myosin light polypeptide 6 

Protein Synonyms/Alias

 17 kDa myosin light chain; LC17; Myosin light chain 3; MLC-3; Myosin light chain alkali 3; Myosin light chain A3; Smooth muscle and nonmuscle myosin light chain alkali 6 

Gene Name

 Myl6 

Gene Synonyms/Alias

 Myln 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
26	FDRTGDGKILYSQCG	ubiquitination	[1]
50	PTNAEVLKVLGNPKS	acetylation	[2, 3, 4]
50	PTNAEVLKVLGNPKS	ubiquitination	[1]
56	LKVLGNPKSDEMNVK	ubiquitination	[1]
63	KSDEMNVKVLDFEHF	ubiquitination	[1]
79	PMLQTVAKNKDQGTY	acetylation	[2]
79	PMLQTVAKNKDQGTY	ubiquitination	[1]
81	LQTVAKNKDQGTYED	acetylation	[2, 3, 4, 5]
81	LQTVAKNKDQGTYED	ubiquitination	[1]
98	EGLRVFDKEGNGTVM	acetylation	[2, 6]
98	EGLRVFDKEGNGTVM	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [6] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379] 

Functional Description

 Regulatory light chain of myosin. Does not bind calcium. 

Sequence Annotation

 DOMAIN 7 42 EF-hand 1.
 DOMAIN 84 119 EF-hand 2.
 DOMAIN 119 151 EF-hand 3.
 MOD_RES 2 2 N-acetylcysteine.
 MOD_RES 81 81 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Motor protein; Muscle protein; Myosin; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 151 AA 

Protein Sequence

Gene Ontology

 GO:0016461; C:unconventional myosin complex; IDA:UniProtKB.
 GO:0030898; F:actin-dependent ATPase activity; ISS:HGNC.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003774; F:motor activity; TAS:HGNC.
 GO:0008307; F:structural constituent of muscle; ISS:HGNC.
 GO:0030049; P:muscle filament sliding; TAS:HGNC.
 GO:0007519; P:skeletal muscle tissue development; TAS:HGNC. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

 PF13405; EF_hand_4 

SMART

 SM00054; EFh 

PROSITE

 PS50222; EF_HAND_2 

PRINTS